Human HOXB13 Antibody

Catalog # Availability Size / Price Qty
AF8156
AF8156-SP
Detection of Human HOXB13 by Western Blot.
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Human HOXB13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HOXB13 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived human HOXB13
Met1-Gln102
Accession # Q92826
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human HOXB13 by Western Blot. View Larger

Detection of Human HOXB13 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HOXB13 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HOXB13 in LNCaP Human Cell Line. View Larger

HOXB13 in LNCaP Human Cell Line. HOXB13 was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HOXB13 in Human Prostate. View Larger

HOXB13 in Human Prostate. HOXB13 was detected in formalin fixed paraffin-embedded sections of human prostate using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXB13

HOXB13 (Homeobox B13) is a member of the homeobox family of transcription factors with a reported molecular weight of approximately 34 kDa. It is 284 amino acids in length and shares 93% aa identity with mouse and rat HOXB13. HOXB13 plays an important role in prostate cancer. Its expression promotes metastatis of prostate cancers and mutations in HOXB13 are associated with early onset disease. Elevated expression of HOXB13 is also associated with aggressive breast cancers, which may be due in part to its ability to reduce the expression of Estrogen Receptor alpha and increase expression of IL-6.

Long Name
Homeobox B13
Entrez Gene IDs
10481 (Human); 15408 (Mouse); 303480 (Rat)
Alternate Names
homeo box B13; homeobox B13; homeobox protein Hox-B13; HOXB13; PSGD

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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