Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HOXB13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human HOXB13
Met1-Gln102
Accession # Q92826

Specificity

Detects human HOXB13 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HOXB13 Antibody

Detection of Human HOXB13 antibody by Western Blot.

Detection of Human HOXB13 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HOXB13 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HOXB13 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

HOXB13 in LNCaP Human Cell Line.

HOXB13 was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
HOXB13 antibody in Human Prostate by Immunohistochemistry (IHC-P).

HOXB13 in Human Prostate.

HOXB13 was detected in formalin fixed paraffin-embedded sections of human prostate using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human HOXB13 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOXB13

HOXB13 (Homeobox B13) is a member of the homeobox family of transcription factors with a reported molecular weight of approximately 34 kDa. It is 284 amino acids in length and shares 93% aa identity with mouse and rat HOXB13. HOXB13 plays an important role in prostate cancer. Its expression promotes metastatis of prostate cancers and mutations in HOXB13 are associated with early onset disease. Elevated expression of HOXB13 is also associated with aggressive breast cancers, which may be due in part to its ability to reduce the expression of Estrogen Receptor alpha and increase expression of IL-6.

Long Name

Homeobox B13

Alternate Names

PSGD

Entrez Gene IDs

10481 (Human); 15408 (Mouse); 303480 (Rat)

Gene Symbol

HOXB13

UniProt

Q92826

Additional HOXB13 Products

Product Documents for Human HOXB13 Antibody

Certificate of Analysis

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Product Specific Notices for Human HOXB13 Antibody

For research use only

Citations for Human HOXB13 Antibody

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Protocols

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