Human IFN-alpha I Antibody

Catalog # Availability Size / Price Qty
MAB10080-100
MAB10080-SP
IFN-  alpha I Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-  alpha I Antibody.
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Human IFN-alpha I Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ alpha I in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 995826
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human IFN‑ alpha I
Met1-Asp189
Accession # P01571
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IFN‑ alpha I inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.6-6 ng/mL in the presence of 20 pg/mL Recombinant Human IFN‑ alpha I.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN-  alpha I Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-  alpha I Antibody. View Larger

IFN- alpha I Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN- alpha I Antibody. Recombinant Human IFN- alpha I reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN- alpha I (20 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN- alpha I Monoclonal Antibody (Catalog # MAB10080). The ND50 is typically 0.6-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha I

Interferon-alpha (IFN-alpha, IFNA), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. There are at least 18 different IFNA subtypes, and the major subtypes identified are IFNA1, IFNA2, IFNA8, IFNA10, IFNA14 and IFNA21.

Long Name
Interferon alpha I
Entrez Gene IDs
3451 (Human)
Alternate Names
IFNA; IFNA17; IFNalpha I; IFN-alpha I; IFN-alpha-17; IFN-alphaI; INFA; interferon alpha-17; Interferon alpha-88; Interferon alpha-I'; Interferon alpha-T; interferon alpha-WA; interferon, alpha 17; LeIF I; LEIF2C1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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