Human IGFLR1 Antibody

Catalog # Availability Size / Price Qty
MAB8296
MAB8296-SP
Detection of IGFLR1 in Human Blood Lymphocytes by Flow Cytometry.
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Human IGFLR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFLR1in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 905338
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human IGFLR1
Met1-Pro163
Accession # Q9H665
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.3 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of rhIGFL-1 to immobilized rhIGFLR1 coated at 100 ng/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGFLR1 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of IGFLR1 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IGFLR1 Monoclonal Antibody (Catalog # MAB8296) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFLR1

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL-1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL-1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

References
  1. Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.
Long Name
IGF-like Family Receptor 1
Entrez Gene IDs
79713 (Human); 101883 (Mouse); 499126 (Rat)
Alternate Names
FLJ22573; IGFLR1; TMEM149; transmembrane protein 149; U2 small nuclear RNA auxiliary factor 1-like 4; U2(RNU2) small nuclear RNA auxiliary factor 1-like 4; U2AF1L4

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