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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    6.5 hours
  • Sensitivity
    0.14 pg/mL
  • Assay Range
    0.125 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma,)
  • Specificity
    Natural and recombinant human IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine HS Human IL-1 beta Immunoassay is a 6.5 hour solid phase ELISA designed to measure human IL-1 beta levels in serum and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum and plasma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested on one plate to assess intra-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean0.2841.7253.2690.3061.6553.195
Standard Deviation0.0290.0960.140.0320.1210.29
CV%10.25.64.310.47.39.1

Recovery

The recovery of IL-1 beta was determined by spiking to levels throughout the range of the assay in various matrices.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 101 87-112
Heparin Plasma (n=4) 103 85-117
Serum (n=4) 103 95-111
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-1 beta and serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
IL
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 150 µL Standard, Control, or Sample
    6.   Add 150 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 6 times.

    11. 50 µL Substrate Solution
    12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker. Do not wash the plate.

    13. 50 µL Amplifier Solution
    14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes on the shaker.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 18
    Filter your results:

    Species
    Sample Type
    1. Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational study.
      Authors: Beghe B, Verduri A, Bottazzi B, Stendardo M, Fucili A, Balduzzi S, Leuzzi C, Papi A, Mantovani A, Fabbri L, Ceconi C, Boschetto P
      PLoS ONE, 2013;8(11):e80166.
      Species: Human
      Sample Type: Plasma
    2. Correlation between salivary IL-1beta levels and periodontal clinical status.
      Authors: Tobon-Arroyave S, Jaramillo-Gonzalez P, Isaza-Guzman D
      Arch Oral Biol, 2008;53(4):346-52.
      Species: Human
      Sample Type: Saliva
    3. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
      Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
      J. Interferon Cytokine Res., 2010;30(11):835-42.
      Species: Human
      Sample Type: Serum
    4. Positive affect and inflammation during radiation treatment for breast and prostate cancer.
      Authors: Sepah SC, Bower JE
      Brain Behav. Immun., 2009;23(8):1068-72.
      Species: Human
      Sample Type: Serum
    5. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
      Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
      Cancer Lett., 2009;283(1):29-35.
      Species: Human
      Sample Type: Serum
    6. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
      Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
      Transplantation, 2009;88(4):582-8.
      Species: Human
      Sample Type: Serum
    7. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
      Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
      J. Exp. Med., 2009;206(5):1029-36.
      Species: Human
      Sample Type: Plasma
    8. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
      Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
      BMC Pulm Med, 2012;12(0):3.
      Species: Human
      Sample Type: Plasma
    9. The influence of anger expression on wound healing.
      Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
      Brain Behav. Immun., 2007;22(5):699-708.
      Species: Human
      Sample Type: Wound Secretion
    10. Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome.
      Authors: Schneider MP, Schlaich MP, Harazny JM, Raff U, Ritt M, Ott C, Schmieder RE
      Obesity (Silver Spring), 2011;19(5):960-7.
      Species: Human
      Sample Type: Plasma
    11. Defining cancer cachexia in head and neck squamous cell carcinoma.
      Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
      Clin. Cancer Res., 2007;13(22):6561-7.
      Species: Human
      Sample Type: Serum
    12. Endothelial activation by platelets from sickle cell anemia patients.
      Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
      PLoS ONE, 2014;9(2):e89012.
    13. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
    14. Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
      Authors: Backstrom E, Lappalainen U, Bry K
      Am. J. Respir. Cell Mol. Biol., 2010;42(2):149-60.
    15. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    16. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    17. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
      Authors: Verma S, Kumar M, Nerurkar VR
      J. Gen. Virol., 2011;92(0):507-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
      Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
      Inflamm. Res., 2010;59(5):339-47.
      Species: Human
      Sample Type: Serum
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