Human IL-17 Quantikine HS ELISA Kit

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Human IL-17 ELISA Standard Curve
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Product Details
Citations (10)
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Human IL-17 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.051 pg/mL
Assay Range
0.2 - 15 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-17
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Human IL-17 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure IL-17 levels in serum and plasma. It contains HEK293-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2
n 20 20
Mean (pg/mL) 2.03 4.09
Standard Deviation 0.055 0.128
CV% 2.7 3.1

Recovery

The recovery of human IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 86-107
Heparin Plasma (n=4) 95 85-107
Serum (n=4) 92 79-105

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-17 ELISA Linearity

Scientific Data

Human IL-17 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 

A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha, IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names:
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-17 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Association of inflammatory biomarkers and disease activity with subclinical myocardial dysfunction in psoriatic arthritis
    Authors: Pletikosic, I;Marasovic Krstulovic, D;Bakovic, D;Susilovic Grabovac, Z;Tandara, L;Martinovic Kaliterna, D;
    Scientific reports
    Species: Human
    Sample Types: Serum
  2. Interleukin-17 contributes to Ross River virus-induced arthritis and myositis
    Authors: H Mostafavi, K Tharmaraja, J Vider, NP West, JR Freitas, B Cameron, PS Foster, LP Hueston, AR Lloyd, S Mahalingam, A Zaid
    PloS Pathogens, 2022-02-10;18(2):e1010185.
    Species: Human
    Sample Types: Serum
  3. The Effect of Three-Month Vitamin D Supplementation on the Levels of Homocysteine Metabolism Markers and Inflammatory Cytokines in Sera of Psoriatic Patients
    Authors: A Prtina, N Rašeta Sim, T Milivojac, M Vujni?, M Grabež, D Djuric, MP Stojiljkov, V Soldat Sta, MJ ?oli?, R Škrbi?
    Biomolecules, 2021-12-11;11(12):.
    Species: Human
    Sample Types: Serum
  4. Evaluating the Role of the Interleukin-23/17 Axis in Critically Ill COVID-19 Patients
    Authors: E Jahaj, AG Vassiliou, C Keskinidou, P Gallos, CS Vrettou, S Tsipilis, Z Mastora, SE Orfanos, I Dimopoulou, A Kotanidou
    Journal of personalized medicine, 2021-09-07;11(9):.
    Species: Human
    Sample Types: Serum
  5. Increased Vdelta1gammadeltaT cells predominantly contributed to IL-17 production in the development of adult human post-infectious irritable bowel syndrome
    Authors: LW Dong, XN Sun, ZC Ma, J Fu, FJ Liu, BL Huang, DC Liang, DM Sun, C Lan
    BMC gastroenterology, 2021-06-30;21(1):271.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Immune Profiling To Predict Outcome of Clostridioides difficile Infection
    Authors: MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri
    MBio, 2020-05-26;11(3):.
    Species: Human
    Sample Types:
  7. New signatures of poor CD4 cell recovery after suppressive antiretroviral therapy in HIV-1-infected individuals: involvement of miR-192, IL-6, sCD14 and miR-144
    Authors: F Hernández-, MJ Ruiz-de-Le, I Rosado-Sán, E Vázquez, M Leal, S Moreno, F Vidal, J Blanco, YM Pacheco, A Vallejo
    Sci Rep, 2020-02-19;10(1):2937.
    Species: Human
    Sample Types: Plasma
  8. Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability
    Authors: HR Ghaiad, AN Elmazny, MM Nooh, MM El-Sawalhi, AA Shaheen
    J Adv Res, 2019-11-04;21(0):141-150.
    Species: Human
    Sample Types: Plasma
  9. IL?1beta increases the expression of inflammatory factors in synovial fluid?derived fibroblast?like synoviocytes via activation of the NF?kappaB?mediated ERK?STAT1 signaling pathway
    Authors: J Yang, J Wang, X Liang, H Zhao, J Lu, Q Ma, B Jing, F Tian
    Mol Med Rep, 2019-10-21;0(0):.
    Species: Rat
    Sample Types: Whole Blood
  10. Elevated regulatory T cells, surface and intracellular CTLA-4 expression and interleukin-17 in the lung cancer microenvironment in humans
    Authors: Joanna Domagala-K
    Cancer Immunol. Immunother., 2016-11-19;0(0):.
    Species: Human
    Sample Types: Serum

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