Interleukin 18 binding protein (IL-18 BP or IL-18 BPa) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BPa bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BPa has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPa share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BPa. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
Human IL‑18 BPa Antibody
R&D Systems | Catalog # MAB1191
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Thr29-Gly192
Accession # AAD17190
Specificity
Clonality
Host
Isotype
Endotoxin Level
Scientific Data Images for Human IL‑18 BPa Antibody
Neutralization of IL-18 BPa Bioactivity by Human IL-18 BPa Antibody
Mouse Anti-Human IL-18 BPa Monoclonal Antibody (Catalog # MAB1191) neutralizes Recombinant Human IL-18 BPa Fc Chimera (119-BP) inhibition of Recombinant Human IL-18/IL-1F4 (9124-IL) induced IFN gamma secretion in the KG-1 human acute myelogenous leukemia cell line as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The ND50 for this effect is typically 0.800-12.0 µg/mL.
Applications for Human IL‑18 BPa Antibody
Neutralization
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-18 BPa
References
- Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
- Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
- Calderara, S. et al. (2001) Virology 279:22.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-18 BPa Products
Product Documents for Human IL‑18 BPa Antibody
Certificate of Analysis
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Product Specific Notices for Human IL‑18 BPa Antibody
For research use only
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