Human IL-18 BPa Antibody

Catalog # Availability Size / Price Qty
MAB1191
MAB1191-SP
IL‑18 BPa Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma  Secretion and Neutralization by Human IL‑18 BPa Antibody.
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Citations (1)
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Human IL-18 BPa Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-18 BPa in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-18 BPc or rmIL-18 BPd is observed.
Source
Monoclonal Mouse IgG1 Clone # 136007
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
Thr29-Gly192
Accession # AAD17190
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑18 BPa inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 µg/mL Recombinant Human IL‑18 BPa Fc Chimera, 40 ng/mL Recombinant Human IL‑18/IL‑1F4, and 20 ng/mL Recombinant Human TNF‑ alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑18 BPa Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma  Secretion and Neutralization by Human IL‑18 BPa Antibody. View Larger

IL‑18 BPa Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Human IL‑18 BPa Antibody. Recombinant Human IL-18 BPa Fc Chimera (Catalog # 119-BP) inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Inhibition of Recombinant Human IL-18/IL-1F4 (40 ng/mL) activity elicited by Recombinant Human IL-18 BPa Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-18 BPa Monoclonal Antibody (Catalog # MAB1191). The ND50 is typically 5-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP or IL-18 BPa) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BPa bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BPa has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPa share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BPa. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

References
  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.
Long Name
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa

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Citation for Human IL-18 BPa Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
    Authors: Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A
    PLoS Pathog, 2014-06-26;10(6):e1004210.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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