Human IL-18 BPa Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-18 BPa in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-18 BPc or rmIL-18 BPd is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 136007
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
    Thr29-Gly192
    Accession # AAD17190
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑18 BPa inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 µg/mL Recombinant Human IL‑18 BPa Fc Chimera, 40 ng/mL Recombinant Human IL‑18/IL‑1F4, and 20 ng/mL Recombinant Human TNF‑ alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑18 BPa Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Human IL‑18 BPa Antibody. Recombinant Human IL‑18 BPa Fc Chimera (Catalog # 119-BP) inhibits Recombinant Human IL‑18/IL‑1F4 induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Inhibition of Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) activity elicited by Recombinant Human IL‑18 BPa Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1191). The ND50 is typically 5‑20 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP or IL-18 BPa) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BPa bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BPa has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPa share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BPa. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

  • References:
    1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
    2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
    3. Calderara, S. et al. (2001) Virology 279:22.
  • Long Name:
    Interleukin 18 Binding Protein Isoform a
  • Entrez Gene IDs:
    10068 (Human)
  • Alternate Names:
    IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
    Authors: Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A
    PLoS Pathog, 2014;10(6):e1004210.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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