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Human IL-1 beta /IL-1F2 Antibody

Catalog #: MAB601
36 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB601-SP
MAB601-100
MAB601-500
Detection of Human IL‑1 beta /IL‑1F2 by Western Blot.
4 Images
Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 2805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
TF‑1 human erythroleukemic cell line
Immunocytochemistry
8-25 µg/mL
See below

Human IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF201)

Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)

Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 0.05 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Product Specific Notices

This product is covered by one or more patents, including US Patent # 5,681,933.

Citations for Human IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
    Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
    Microbial pathogenesis, 2023;180(0):106120.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Oral Administration of Clostridium butyricum GKB7 Ameliorates Signs of Osteoarthritis in Rats
    Authors: SL Chang, YY Lin, SC Liu, YS Tsai, SW Lin, YL Chen, CC Chen, CY Ko, HT Chen, WC Chen, CH Tang
    Cells, 2022;11(14):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Therapeutic Effects of Live Lactobacillus plantarum GKD7 in a Rat Model of Knee Osteoarthritis
    Authors: YY Lin, SL Chang, SC Liu, D Achudhan, YS Tsai, SW Lin, YL Chen, CC Chen, JW Chang, YC Fong, SL Hu, CH Tang
    Nutrients, 2022;14(15):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. The central inflammasome adaptor protein ASC activates the inflammasome after transition from a soluble to an insoluble state
    Authors: ER Prather, MA Gavrilin, MD Wewers
    The Journal of Biological Chemistry, 2022;0(0):102024.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA (Capture)
  5. Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells
    Authors: JFS Pereira, C Bessa, P Matos, P Jordan
    Cancers, 2022;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. 1,25-Dihydroxyvitamin D3 attenuates IL-1beta secretion by suppressing NLRP1 inflammasome activation by upregulating the NRF2-HO-1 pathway in epidermal keratinocytes
    Authors: T Nakajo, T Katayoshi, N Kitajima, K Tsuji-Nait
    Redox Biology, 2021;48(0):102203.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
    Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
    PLoS Pathog., 2020;16(8):e1008781.
    Species: Human
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  8. The IL1beta-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs)
    Authors: R Lappano, M Talia, F Cirillo, DC Rigiraccio, D Scordamagl, R Guzzi, AM Miglietta, EM De Frances, A Belfiore, AH Sims, M Maggiolini
    J. Exp. Clin. Cancer Res., 2020;39(1):153.
    Species: Human
    Sample Types: Cell Culture Lysates, Cell Lysates
    Applications: Western Blot
  9. Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model
    Authors: E Tarricone, E Mattiuzzo, E Belluzzi, R Elia, A Benetti, R Venerando, V Vindigni, P Ruggieri, P Brun
    Cells, 2020;9(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis
    Authors: BD Bryson, TR Rosebrock, FG Tafesse, CY Itoh, A Nibasumba, GH Babunovic, B Corleis, C Martin, C Keegan, P Andrade, S Realegeno, D Kwon, RL Modlin, SM Fortune
    Nat Commun, 2019;10(1):2329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
    Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
    Arch. Biochem. Biophys., 2019;0(0):108238.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion
    Authors: Z Li, G Wang, D Feng, G Zu, Y Li, X Shi, Y Zhao, H Jing, S Ning, W Le, J Yao, X Tian
    Cell Death Dis, 2018;9(5):483.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans
    Authors: K Kuranda, P Jean-Alpho, C Leborgne, R Hardet, F Collaud, S Marmier, H Costa Verd, G Ronzitti, P Veron, F Mingozzi
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
    Authors: L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung
    J. Allergy Clin. Immunol., 2018;0(0):.
  15. Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages
    Authors: T Karasawa, A Kawashima, F Usui-Kawan, S Watanabe, H Kimura, R Kamata, K Shirasuna, Y Koyama, A Sato-Tomit, T Matsuzaka, H Tomoda, SY Park, N Shibayama, H Shimano, T Kasahara, M Takahashi
    Arterioscler. Thromb. Vasc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia
    Authors: DC Johnson, CY Taabazuing, MC Okondo, AJ Chui, SD Rao, FC Brown, C Reed, E Peguero, E de Stanchi, A Kentsis, DA Bachovchin
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
    Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
    PLoS Pathog., 2017;13(9):e1006577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
    Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
    Authors: Rahman M, Mitra S, Sarkar A, Wewers M
    PLoS ONE, 2015;10(2):e0117330.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  20. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015;10(6):e0130395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
    Authors: Ghonime M, Shamaa O, Das S, Eldomany R, Fernandes-Alnemri T, Alnemri E, Gavrilin M, Wewers M
    J Immunol, 2014;192(8):3881-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  22. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
    Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
    Nat. Immunol., 2011;12(12):1159-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  24. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  25. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  26. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008;24(5):667-71.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: ELISA Development
  27. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  28. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  29. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  30. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  31. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  32. HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta.
    Authors: Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J
    Glia, 2006;54(6):619-29.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  33. Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
    Authors: Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
    J. Periodontol., 2006;77(10):1695-700.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
    Applications: ELISA Development
  34. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  35. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  36. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: ELISA Development

FAQs

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Reviews for Human IL-1 beta /IL-1F2 Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 08/12/2021
Application: WB Sample Tested: microglial cells Species: Human
Western Blot Human IL-1 beta /IL-1F2 Antibody MAB601
Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human IL-1 beta /IL-1F2 Antibody MAB601
Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human