Human IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
MAB601-SP
MAB601-100
MAB601-500
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot.
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Citations (63)
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Human IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 2805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
TF‑1 human erythroleukemic cell line
Immunocytochemistry
8-25 µg/mL
See below

Human IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF201)

Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)

Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 0.05 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western View Larger

Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL.

Detection of Human IL-1 beta/IL-1F2 by ELISA Effect of A1AT on whole blood IL-1 beta release. IL-1 beta production in whole blood cultures in response to LPS (1.0 μg/ml) was performed in the presence of endogenous A1AT (i.e., undiluted) or exogenously added A1AT (2 mg/ml) in blood diluted 1:32 with RPMI. Whole blood cultures were incubated for 18 h. After incubation, plasma supernatants were removed, and IL-1 beta quantified by ELISA and expressed as mean ± SD for three donors. The diluted sample result was corrected for the dilution. NS indicates no significant difference. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117330), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot Effect of high glucose and IL-1 beta alone or in combination on the protein expressions of CD31, FSP1 and alpha -SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for hyperosmolarity. Representative western blots (a) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (b–d) are presented. e–h HAECs were treated for 48 h with NG, HG, IL-1 beta (10 ng/ml)and HG in the presence of the IL-1 beta (10 ng/ml). Representative western blots (E) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (f–h) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol, IL-1 beta (10 ng/ml), HG + IL-1 beta : high glucose (30 mM) + IL-1 beta (10 ng/ml) *P < 0.05 vs. MN or NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot Effects of PKC beta on high glucose induced IL-1 beta up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKC beta inhibitors (LY317615, 0.3 μM) for 48 h. Real-time PCR analyses showed mRNA expression of PKC beta and IL-1 beta (a, b). Representative western blots (c) and quantitative determinations of PKC beta and IL-1 beta (d, e) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), PMA (30 nM): phorbol 12-myristate13-acetate; LY (0.3 uM): LY317615; *P < 0.05 vs.NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG or PMA Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot The influence of blocking IL-1 beta treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1 beta. (a–f) HAECs were incubated for 48 h with anti-IL-1 beta antibodies (1000 ng/ml) in the presence of NG or HG. (a1–f1) We performed gene-silencing experiments using transfection with siRNA specific for IL-1 beta. The protein expressions of IL-1 beta, CD31, FSP1 and alpha -SMA were assessed by western blotting. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM). Anti-IL-1 beta : anti-IL-1 beta antibodies (1000 ng/ml). *P < 0.05 vs. NG or anti-IL-1 beta, #P < 0.05 vs. HG or HG +Vehicle Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Product Specific Notices

This product is covered by one or more patents, including US Patent # 5,681,933.

Citations for Human IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

63 Citations: Showing 1 - 10
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  1. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
    Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
    Frontiers in Immunology
  2. Identification of Predictive Early Biomarkers for Sterile-SIRS after Cardiovascular Surgery.
    Authors: Stoppelkamp S, Veseli K, Stang K, Schlensak C, Wendel H, Walker T
    PLoS ONE, 2015-08-11;10(8):e0135527.
  3. Glucose Induces IL-1 alpha -Dependent Inflammation and Extracellular Matrix Proteins Expression and Deposition in Renal Tubular Epithelial Cells in Diabetic Kidney Disease
    Authors: Talal Salti, Khaled Khazim, Rami Haddad, Salvatore Campisi-Pinto, Gil Bar-Sela, Idan Cohen
    Frontiers in Immunology
  4. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways
    Authors: Lai J, Wu D, Wu C et al.
    iScience
  5. Vitamin D Induces Interleukin-1b Expression: Paracrine Macrophage Epithelial Signaling Controls M. tuberculosis Infection.
    Authors: Verway M, Bouttier M, Wang TT et al.
    PLoS Pathog.
  6. miR-146a-5p plays an essential role in the aberrant epithelial-fibroblast cross-talk in COPD
    Authors: ET Osei, L Florez-Sam, H Tasena, A Faiz, JA Noordhoek, W Timens, DS Postma, TL Hackett, IH Heijink, CA Brandsma
    Eur. Respir. J., 2017-05-25;49(5):.
  7. Inflammasome priming is similar for francisella species that differentially induce inflammasome activation.
    Authors: Ghonime MG, Mitra S, Eldomany RA et al.
    PLoS ONE.
  8. Tumor Necrosis Factor and Interleukin-1 beta Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation
    Authors: Ishitoku, M;Mokuda, S;Araki, K;Watanabe, H;Kohno, H;Sugimoto, T;Yoshida, Y;Sakaguchi, T;Masumoto, J;Hirata, S;Sugiyama, E;
    Viruses
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
    Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
    Microbial pathogenesis, 2023-04-18;180(0):106120.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  10. Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection
    Authors: Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al.
    ACS Nano
  11. Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
    Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
    Microbial pathogenesis, 2023;180(0):106120.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  12. Therapeutic Effects of Live Lactobacillus plantarum GKD7 in a Rat Model of Knee Osteoarthritis
    Authors: YY Lin, SL Chang, SC Liu, D Achudhan, YS Tsai, SW Lin, YL Chen, CC Chen, JW Chang, YC Fong, SL Hu, CH Tang
    Nutrients, 2022-08-01;14(15):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Oral Administration of Clostridium butyricum GKB7 Ameliorates Signs of Osteoarthritis in Rats
    Authors: SL Chang, YY Lin, SC Liu, YS Tsai, SW Lin, YL Chen, CC Chen, CY Ko, HT Chen, WC Chen, CH Tang
    Cells, 2022-07-11;11(14):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. The central inflammasome adaptor protein ASC activates the inflammasome after transition from a soluble to an insoluble state
    Authors: ER Prather, MA Gavrilin, MD Wewers
    The Journal of Biological Chemistry, 2022-05-11;0(0):102024.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA (Capture)
  15. Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells
    Authors: JFS Pereira, C Bessa, P Matos, P Jordan
    Cancers, 2022-03-09;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Maternal Cigarette Smoke Exposure Exaggerates the Behavioral Defects and Neuronal Loss Caused by Hypoxic-Ischemic Brain Injury in Female Offspring
    Authors: Taida Huang, Xiaomin Huang, Hui Li, Junhua Qi, Nan Wang, Yi Xu et al.
    Frontiers in Cellular Neuroscience
  17. 1,25-Dihydroxyvitamin D3 attenuates IL-1beta secretion by suppressing NLRP1 inflammasome activation by upregulating the NRF2-HO-1 pathway in epidermal keratinocytes
    Authors: T Nakajo, T Katayoshi, N Kitajima, K Tsuji-Nait
    Redox Biology, 2021-12-01;48(0):102203.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Machine learning identifies molecular regulators and therapeutics for targeting SARS‐CoV2‐induced cytokine release
    Authors: Marina Chan, Siddharth Vijay, John McNevin, M Juliana McElrath, Eric C Holland, Taranjit S Gujral
    Molecular Systems Biology
  19. Tumor‐associated macrophages promote the metastasis and growth of non‐small‐cell lung cancer cells through NF‐ kappa B/PP2Ac‐positive feedback loop
    Authors: Zhan‐Wen Liang, Xin‐Xin Ge, Meng‐Dan Xu, Hualong Qin, Meng‐Yao Wu, Meng Shen et al.
    Cancer Science
  20. Non-canonical roles of caspase-4 and caspase-5 in heme driven- IL-1 beta release and cell death
    Authors: Beatriz E. Bolívar, Alexandra N. Brown-Suedel, Brittany A. Rohrman, Chloé I. Charendoff, Vanda Yazdani, John D. Belcher et al.
    The Journal of Immunology
  21. Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1 alpha /Glycolysis-Dependent Axis
    Authors: Ana Campos Codo, Gustavo Gastão Davanzo, Lauar de Brito Monteiro, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, João Victor Virgilio-da-Silva et al.
    Cell Metabolism
  22. Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
    Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
    PLoS Pathog., 2020-08-18;16(8):e1008781.
    Species: Human
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  23. The IL1beta-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs)
    Authors: R Lappano, M Talia, F Cirillo, DC Rigiraccio, D Scordamagl, R Guzzi, AM Miglietta, EM De Frances, A Belfiore, AH Sims, M Maggiolini
    J. Exp. Clin. Cancer Res., 2020-08-10;39(1):153.
    Species: Human
    Sample Types: Cell Culture Lysates, Cell Lysates
    Applications: Western Blot
  24. Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model
    Authors: E Tarricone, E Mattiuzzo, E Belluzzi, R Elia, A Benetti, R Venerando, V Vindigni, P Ruggieri, P Brun
    Cells, 2020-05-26;9(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
    Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
    Arch. Biochem. Biophys., 2019-12-24;0(0):108238.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Inhibition of IRE1 alpha RNase activity reduces NLRP3 inflammasome assembly and processing of pro-IL1 beta
    Authors: Aaron Talty, Shane Deegan, Mila Ljujic, Katarzyna Mnich, Serika D. Naicker, Dagmar Quandt et al.
    Cell Death & Disease
  27. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis
    Authors: BD Bryson, TR Rosebrock, FG Tafesse, CY Itoh, A Nibasumba, GH Babunovic, B Corleis, C Martin, C Keegan, P Andrade, S Realegeno, D Kwon, RL Modlin, SM Fortune
    Nat Commun, 2019-05-27;10(1):2329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Structural Modification of the Antidepressant Mianserin Suggests That Its Anti-inflammatory Activity May Be Independent of 5-Hydroxytryptamine Receptors
    Authors: Sandra Sacre, Albert Jaxa-Chamiec, Caroline M. R. Low, Giselle Chamberlain, Cathy Tralau-Stewart
    Frontiers in Immunology
  29. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans
    Authors: K Kuranda, P Jean-Alpho, C Leborgne, R Hardet, F Collaud, S Marmier, H Costa Verd, G Ronzitti, P Veron, F Mingozzi
    J. Clin. Invest., 2018-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia
    Authors: DC Johnson, CY Taabazuing, MC Okondo, AJ Chui, SD Rao, FC Brown, C Reed, E Peguero, E de Stanchi, A Kentsis, DA Bachovchin
    Nat. Med., 2018-07-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion
    Authors: Z Li, G Wang, D Feng, G Zu, Y Li, X Shi, Y Zhao, H Jing, S Ning, W Le, J Yao, X Tian
    Cell Death Dis, 2018-05-01;9(5):483.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages
    Authors: T Karasawa, A Kawashima, F Usui-Kawan, S Watanabe, H Kimura, R Kamata, K Shirasuna, Y Koyama, A Sato-Tomit, T Matsuzaka, H Tomoda, SY Park, N Shibayama, H Shimano, T Kasahara, M Takahashi
    Arterioscler. Thromb. Vasc. Biol., 2018-02-08;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Calcium phosphate particles stimulate interleukin-1 beta release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release
    Authors: Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook et al.
    Journal of Molecular and Cellular Cardiology
  34. ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
    Authors: L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung
    J. Allergy Clin. Immunol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralizing
  35. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
    Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
    PLoS Pathog., 2017-09-01;13(9):e1006577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
    Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
    Mol Med Rep, 2017-08-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Interleukin-1 beta mediates high glucose induced phenotypic transition in human aortic endothelial cells
    Authors: Dong-Dong Zhu, Ri-Ning Tang, Lin-Li Lv, Yi Wen, Hong Liu, Xiao-Liang Zhang et al.
    Cardiovascular Diabetology
  38. Interleukin-1 alpha drives the dysfunctional cross-talk of the airway epithelium and lung fibroblasts in COPD
    Authors: Emmanuel T. Osei, Jacobien A. Noordhoek, Tillie L. Hackett, Anita I.R. Spanjer, Dirkje S. Postma, Wim Timens et al.
    European Respiratory Journal
  39. Offloading treatment is linked to activation of proinflammatory cytokines and start of bone repair and remodeling in Charcot arthropathy patients
    Authors: Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al.
    Journal of Foot and Ankle Research
  40. Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
    Authors: Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille
    BMC Biotechnology
  41. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015-06-24;10(6):e0130395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  42. Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
    Authors: Rahman M, Mitra S, Sarkar A, Wewers M
    PLoS ONE, 2015-02-06;10(2):e0117330.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  43. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation.
    Authors: Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, Timmel T, Bullock A, Knaus P
    J Biol Chem, 2014-11-03;290(6):3390-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  44. Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
    Authors: Ghonime M, Shamaa O, Das S, Eldomany R, Fernandes-Alnemri T, Alnemri E, Gavrilin M, Wewers M
    J Immunol, 2014-03-12;192(8):3881-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  45. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
    Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
    Nat. Immunol., 2011-10-12;12(12):1159-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  46. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 2011-09-01;26(9):1132-9.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  47. Correlations between both the expression levels of inflammatory mediators and growth factor in medial perimeniscal synovial tissue and the severity of medial knee osteoarthritis
    Authors: Liang Ning, Muneaki Ishijima, Haruka Kaneko, Hidetake Kurihara, Eri Arikawa-Hirasawa, Mitsuaki Kubota et al.
    International Orthopaedics
  48. Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop
    Authors: Florina Olaru, Liselotte E. Jensen
    Journal of Investigative Dermatology
  49. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  50. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  51. MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8
    Authors: Dipa Bhaumik, Gary K. Scott, Shiruyeh Schokrpur, Christopher K. Patil, Arturo V. Orjalo, Francis Rodier et al.
    ImpactJ
  52. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008-07-01;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  53. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: ELISA Development
  54. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  55. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  56. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  57. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007-02-28;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  58. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  59. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  60. HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta.
    Authors: Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J
    Glia, 2006-11-01;54(6):619-29.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  61. Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
    Authors: Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
    J. Periodontol., 2006-10-01;77(10):1695-700.
    Species: Human
    Sample Types:
    Applications: ELISA Development
  62. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  63. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development

FAQs

  1. Can pro-IL-1 beta be detected by Catalog #s AF-201-NA and MAB601 ?

    • The amino acid sequence present in mature IL-1 beta (Ala117-Ser269) is also present in pro IL-1 beta. So in theory, both Catalog #s AF-201-NA and MAB601 should detect the pro form of IL-1 beta. The results would depend on whether the pro form is abundantly present in the sample type being evaluated.

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Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 08/12/2021
Application: WB Sample Tested: microglial cells Species: Human

Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human