Human IL-2 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC202P
Human IL-2 PE-conjugated Antibody in Data
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Product Details
Citations (5)
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Human IL-2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
Source
Monoclonal Mouse IgG1 Clone # 5334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL‑2 in Human PBMCs by Flow Cytometry. View Larger

Detection of IL‑2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and ionomycin were stained with Mouse Anti-Human IL-2 PE-conjugated Monoclonal Antibody (Catalog # IC202P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Citations for Human IL-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
    Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
    J Immunol, 2014;192(1):123-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Building and optimizing a virus-specific T cell receptor library for targeted immunotherapy in viral infections.
    Authors: Banu, Nasirah, Chia, Adeline, Ho, Zi Zong, Garcia, Alfonso, Paravasivam, Komathi, Grotenbreg, Gijsbert, Bertoletti, Antonio, Gehring, Adam J
    Sci Rep, 2014;4(0):4166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2012;6(1):e16195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
    Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
    Cancer Res., 2007;67(20):9630-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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