Human IL-2 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
  • Source
    Monoclonal Mouse IgG1 Clone # 5334
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑2
    Ala21-Thr153
    Accession # NP_000577
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin
  • Neutralization
    Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.
Immunocytochemistry
IL‑2 in Human PBMCs.
IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2
Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016;6(0):32320.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Activin A programs the differentiation of human TFH cells
    Nat Immunol, 2016;17(8):976-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015;290(37):22338-51.
    Species: Human
    Sample Type: Protein
    Application: Neut
  4. Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells.
    Authors: Kim, Dae Won, Zloza, Andrew, Broucek, Joseph, Schenkel, Jason M, Ruby, Carl, Samaha, Georges, Kaufman, Howard L
    J Transl Med, 2014;12(0):113.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012;119(26):6268-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
    Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
    Vaccine, 2010;28(8):1932-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007;109(11):4856-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
    Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
    Cancer Res., 2005;65(18):8101-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003;171(10):5034-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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