Human IL-21 R APC-conjugated Antibody

Clone 152512 was used by HLDA to establish CD designation.
(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human IL‑2 R beta or recombinant mouse IL‑21 R.
  • Source
    Monoclonal Mouse IgG1 Clone # 152512
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R
    Accession # Q9HBE5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑21 R in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL‑21 R APC‑conjugated Monoclonal Antibody (Catalog # FAB9911A) and Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-21 R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also known as NILR (Novel Interleukin Receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The Common gamma  Chain ( gamma c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

  • References:
    1. Parrish-Novak, et al. (2000) Nature 408:57.
    2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
    3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
    4. Asao, H. et al. (2001) J. Immunol. 167:1.
  • Long Name:
    Interleukin 21 Receptor
  • Entrez Gene IDs:
    50615 (Human); 60504 (Mouse)
  • Alternate Names:
    CD360 antigen; CD360; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis.
    Authors: Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S
    J Immunol, 2015;194(11):5139-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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