Human IL-23R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB14001P-100
FAB14001P-025
Detection of IL‑23 R in Human PBMCs by Flow Cytometry.
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Product Details
Citations (12)
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Supplemental Products
Reviews (1)

Human IL-23R PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑23 R.
Source
Monoclonal Mouse IgG2B Clone # 218213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-23 R
Gly24-Ile354
Accession # Q5VWK5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of IL‑23 R in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) cultured with plate-bound 5 μg/mL anti-Human CD3, 2 μg/mL anti-Human CD28 (AF-342-PB), 20 ng/mL Recombinant Human IL-2 (202-GMP), 10 ng/mL TGF-beta (100-B), 20 ng/mL Recombinant Human IL-23 (1290-IL), 40 ng/mL Recombinant Human IL-6 (206-IL), 10 ng/mL Recombinant Human IL-1 beta (201-LB), and 10 μg/mL anti-Human IFN-gamma (AF-285-NA) for 7 days to induce Th17 development or (B) resting, were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A) and Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P). Quadrant markers were set based on Mouse IgG2B Isotype Control (IC0041P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry View Larger

Detection of IL‑23 R in HEK293 cell line transfected with human IL-23 R by Flow Cytometry. HEK293 human cell line transfected with human IL-23 R were stained with Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P, filled histogram) or Mouse IgG2B Isotype Control (IC0041P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5448.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23 Receptor
Entrez Gene IDs
149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)
Alternate Names
IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

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Citations for Human IL-23R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. In Vitro Cellular and Molecular Interplay between Human Foreskin-Derived Mesenchymal Stromal/Stem Cells and the Th17 Cell Pathway
    Authors: M Najar, M Merimi, WH Faour, CA Lombard, D Moussa Agh, Y Ouhaddi, EM Sokal, L Lagneaux, H Fahmi
    Pharmaceutics, 2021-10-19;13(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients.
    Authors: Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A
    Mediators Inflamm, 2020-02-19;2020(0):6401969.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Role of interleukin-23 in monocyte-derived dendritic cells of HBV-related acute-on-chronic liver failure and its correlation with the severity of liver damage
    Authors: Suxia Bao
    Clin Res Hepatol Gastroenterol, 2016-12-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity
    Authors: Lars Fugger
    Sci Transl Med, 2016-11-02;8(363):363ra149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
    Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
    Eur J Immunol, 2015-02-01;45(2):442-51.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015-01-19;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  7. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012-09-25;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2011-12-23;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
    Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
    J. Immunol., 2011-03-02;186(7):4175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2010-10-15;134(2):113-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
    Authors: de Paus RA, van de Wetering D, Van Dissel JT
    Mol. Immunol., 2008-09-01;45(15):3889-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human IL‑23 R PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100