Human IL-4I1 Antibody Summary
Accession # Q96RQ9
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IL‑4I1 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM of PMA for 24 hours followed by 10 μg/mL of LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB56843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-4I1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of IL-4l1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human cell line was stained with Mouse Anti-Human IL-4l1 Monoclonal Antibody (Catalog # MAB56843, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H2O2 production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).
- Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
- Mason, J.M. et al. (2004) J. Immunol. 173:4561.
- Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
- Copie-Bergman, C. et al. (2003) Blood 101:2756.
- Boulland, M.L. et al. (2007) Blood 110:220.
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