Human IL-6 High Sensitivity Magnetic Luminex Performance Assay

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Human IL-6 High Sensitivity Magnetic Luminex Performance Assay Summary

Panel Luminex Performance Human High Sensitivity Cytokine Magnetic Panel A
Luminex Performance Human High Sensitivity Cytokine Magnetic Panel B
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

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Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human IL-6 with up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Magnetic beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor.

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Human IL-6 High Sensitivity Magnetic Luminex Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. The effect of acute exercise on interleukin-6 and hypothalamic-pituitary-adrenal axis responses in patients with coronary artery disease
    Authors: Z Mahmood, A Davidsson, E Olsson, P Leanderson, AK Lundberg, L Jonasson
    Scientific Reports, 2020;10(1):21390.
    Species: Human
    Sample Types: Plasma
  2. Moderators for depressed mood and systemic and transcriptional inflammatory responses: a randomized controlled trial of endotoxin
    Authors: MR Irwin, S Cole, R Olmstead, EC Breen, JJ Cho, M Moieni, NI Eisenberge
    Neuropsychopharmacology, 2019;44(3):635-641.
    Species: Human
    Sample Types: Plasma
  3. Patients with idiopathic recurrent miscarriage have abnormally high TGF�+ blood NK, NKT and T cells in the presence of abnormally low TGF� plasma levels
    Authors: L Zhu, M Aly, RJ Kuon, B Toth, H Wang, H Karakizlis, R Weimer, C Morath, E Ibrahim, N Ekpoom, G Opelz, V Daniel
    BMC Immunol., 2019;20(1):10.
    Species: Human
    Sample Types: Plasma
  4. Transforming growth factor-beta profiles correlate with clinical symptoms and parameters of haemostasis and inflammation in a controlled human malaria infection
    Authors: GM de Jong, MBB McCall, WA Dik, RT Urbanus, LJ Wammes, R Koelewijn, RW Sauerwein, A Verbon, JJ van Hellem, PJJ van Gender
    Cytokine, 2019;125(0):154838.
    Species: Human
    Sample Types: Serum
  5. Cytokines in relation to hCG are significantly altered in asymptomatic women with miscarriage - a pilot study
    Authors: A Freis, J Schlegel, V Daniel, J Jauckus, T Strowitzki, A Germeyer
    Reprod. Biol. Endocrinol., 2018;16(1):93.
    Species: Human
    Sample Types: Plasma
  6. ?-3 fatty acids-supplementary in gestation alleviates neuroinflammation and modulates neurochemistry in rats
    Authors: M Tang, R Dang, S Liu, M Zhang, Y Zheng, R Yang, T Yin
    Lipids Health Dis, 2018;17(1):247.
    Species: Rat
    Sample Types: Serum
  7. The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA
    Authors: AM Quinn, AR Williams, TI Sivilli, CL Raison, TWW Pace
    Stress, 2018;0(0):1-6.
    Species: Human
    Sample Types: Plasma
  8. Exposure to an inflammatory challenge enhances neural sensitivity to negative and positive social feedback
    Authors: Naomi I Eisenberge
    Brain Behav. Immun., 2016;57(0):21-9.
    Species: Human
    Sample Types: Plasma
  9. Cytokines and Postoperative Delirium in Older Patients Undergoing Major Elective Surgery.
    Authors: Vasunilashorn S, Ngo L, Inouye S, Libermann T, Jones R, Alsop D, Guess J, Jastrzebski S, McElhaney J, Kuchel G, Marcantonio E
    J Gerontol A Biol Sci Med Sci, 2015;0(0):.
    Species: Human
    Sample Types: Plasma
  10. Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma.
    Authors: Beane J, Lee G, Zheng Z, Mendel M, Abate-Daga D, Bharathan M, Black M, Gandhi N, Yu Z, Chandran S, Giedlin M, Ando D, Miller J, Paschon D, Guschin D, Rebar E, Reik A, Holmes M, Gregory P, Restifo N, Rosenberg S, Morgan R, Feldman S
    Mol Ther, 2015;23(8):1380-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
    Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
    PLoS ONE, 2014;9(2):e89341.
    Species: Human
    Sample Types: Plasma

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