Human IL-6 Quantikine HS ELISA

R&D Systems | Catalog # HS600B

R&D Systems
Discontinued Product
HS600B has been discontinued. An alternative/replacement product is available: HS600C. View all IL-6 products.

Key Product Details

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Serum (100 µL), EDTA Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)

Sensitivity

0.11 pg/mL

Assay Range

0.156-10 pg/mL (Serum, EDTA Plasma, Citrate Plasma, Urine)
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Product Summary for Human IL-6 Quantikine HS ELISA

The Quantikine HS Human IL-6 Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human IL-6 in serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-6 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-6.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-6

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Citrate Plasma, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 36 36 36
Mean (pg/mL) 0.436 2.45 5.53 0.490 2.78 5.65
Standard Deviation 0.030 0.19 0.41 0.047 0.20 0.37
CV% 6.9 7.8 7.4 9.6 7.2 6.5

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 35 35 35
Mean (pg/mL) 0.526 3.09 5.77 0.502 2.78 5.62
Standard Deviation 0.052 0.18 0.32 0.056 0.19 0.31
CV% 9.8 5.8 5.5 11.2 6.8 5.5

Recovery for Human IL-6 Quantikine HS ELISA

The recovery of IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=8) 106 90-125
EDTA Plasma (n=8) 97 84-113
Serum (n=8) 94 87-99
Urine (n=8) 102 93-112

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-6 High Sensitivity ELISA Linearity
Human IL-6 High Sensitivity ELISA Linearity

Scientific Data Images for Human IL-6 Quantikine HS ELISA

Human IL-6 High Sensitivity ELISA Assay

Human IL-6 High Sensitivity ELISA Assay

Human IL-6 ELISA Serum/Plasma Assay

Human IL-6 ELISA Serum/Plasma Assay

Human IL-6 ELISA Urine Assay

Human IL-6 ELISA Urine Assay

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.


R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filters above to drill down to the IL-6 product that best fits your needs.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Human IL-6 Quantikine HS ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-6 Quantikine HS ELISA

For research use only

Citations for Human IL-6 Quantikine HS ELISA

Customer Reviews for Human IL-6 Quantikine HS ELISA (7)

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Showing  1 - 5 of 7 reviews Showing All
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  • Human IL-6 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 01/20/2020
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Leslie Priddy
    Sample Tested: Serum
    Verified Customer | Posted 03/30/2018
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Balaji Mahender
    Sample Tested: Heparin Plasma, EDTA Plasma and Citrate Plasma
    Verified Customer | Posted 01/18/2018
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 05/03/2017
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Elizabeth Belonga
    Sample Tested: Serum
    Verified Customer | Posted 04/24/2017
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Francesco Marino
    Sample Tested: Serum
    Verified Customer | Posted 11/04/2016
    Great and easy to use kit
  • Human IL-6 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Buffer: Kit dilution buffer<br />Dilution: 1/100
    Human IL-6 Quantikine HS ELISA HS600B

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FAQs for Human IL-6 Quantikine HS ELISA

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  • Q: What is the normal appearance of Assay Diluent RD1-75?

    A: Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

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