Human IL-6 R alpha Quantikine ELISA Kit

  (33 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (33)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    15.1 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human IL-6 sR
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC05 , Quantikine Immunoassay Control Group 2 - Please Inquire
Product Summary
The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 134 644 1669 130 645 1593
Standard Deviation 11.5 16.8 38.3 8.3 26.9 75.4
CV% 8.6 2.6 2.3 6.4 4.2 4.7

Recovery

The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-97
Citrate Plasma (n=4) 93 86-102
EDTA Plasma (n=4) 93 76-107
Heparin Plasma (n=4) 101 87-118
Serum (n=4) 93 86-110
Urine (n=4) 86 64-98
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of natural or recombinant human IL-6 sR were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the as
 IL-6 R alpha [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6 R alpha
IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.
  • Long Name:
    Interleukin 6 Receptor alpha
  • Entrez Gene IDs:
    3570 (Human); 16194 (Mouse)
  • Alternate Names:
    CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Sample Type
  1. Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner
    Authors: H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas
    Authors: Koji Osuka
    J. Neurotrauma, 2016;0(0):.
    Species: Human
    Sample Type: Chronic Subdural Hematoma Fluids
  3. Prevalent peripheral arterial disease and inflammatory burden
    BMC Geriatr, 2016;16(1):213.
    Species: Human
    Sample Type: Serum
  4. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS)
    J Bone Miner Res, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  6. A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors.
    Authors: Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R
    Cancer Res, 2016;76(2):480-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction.
    Authors: Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A
    PLoS ONE, 2015;10(3):e0119415.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
    Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
    Blood, 2014;123(5):650-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013;10(0):29.
    Species: Human
    Sample Type: Serum
  10. Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
    Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
    J. Bone Miner. Res., 2012;27(5):1167-76.
    Species: Human
    Sample Type: Serum
  11. Bruton tyrosine kinase inhibition is a novel therapeutic strategy targeting tumor in the bone marrow microenvironment in multiple myeloma.
    Authors: Tai Y, Chang B, Kong S, Fulciniti M, Yang G, Calle Y, Hu Y, Lin J, Zhao J, Cagnetta A, Cea M, Sellitto M, Zhong M, Wang Q, Acharya C, Carrasco D, Buggy J, Elias L, Treon S, Matsui W, Richardson P, Munshi N, Anderson K
    Blood, 2012;120(9):1877-87.
    Species: Human
    Sample Type: Recombinant Protein
  12. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010;162(3):500-9.
    Species: Human
    Sample Type: Milk
  13. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
    Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
    Cancer Cell, 2009;16(4):309-23.
    Species: Human
    Sample Type: Serum
  14. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009;69(1):329-37.
    Species: Human
    Sample Type: Serum
  15. Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease.
    Authors: Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A
    Int. J. Cancer, 2009;124(2):367-75.
    Species: Human
    Sample Type: Plasma
  16. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Type: Urine
  17. Daily family assistance and inflammation among adolescents from Latin American and European backgrounds.
    Authors: Fuligni AJ, Telzer EH, Bower J, Irwin MR, Kiang L, Cole SW
    Brain Behav. Immun., 2009;23(6):803-9.
    Species: Human
    Sample Type: Plasma
  18. Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers.
    Authors: Shariat SF, Karam JA, Walz J, Roehrborn CG, Montorsi F, Margulis V, Saad F, Slawin KM, Karakiewicz PI
    Clin. Cancer Res., 2008;14(12):3785-91.
    Species: Human
    Sample Type: Plasma
  19. Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma.
    Authors: Egler RA, Burlingame SM, Nuchtern JG, Russell HV
    Clin. Cancer Res., 2008;14(21):7028-34.
    Species: Human
    Sample Type: Plasma
  20. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
    Species: Human
    Sample Type: Plasma
  21. Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
    Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
    Blood, 2007;110(6):1748-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
    Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
    J. Bone Miner. Res., 2007;22(7):1088-95.
    Species: Human
    Sample Type: Serum
  23. Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
    Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
    Arthritis Rheum., 2006;54(5):1662-72.
    Species: Human
    Sample Type: Serum
  24. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005;99(4):411-8.
    Species: Human
    Sample Type: Synovial Fluid
  25. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Type: Plasma
  26. Effect of influenza vaccine on markers of inflammation and lipid profile.
    Authors: Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK
    J. Lab. Clin. Med., 2005;145(6):323-7.
    Species: Human
    Sample Type: Plasma
  27. Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
    Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
    Heart Vessels, 2004;19(5):237-41.
    Species: Human
    Sample Type: Plasma
  28. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Serum
  29. Effects of IL-6 variants in multiple myeloma: growth inhibition and induction of apoptosis in primary cells.
    Authors: Petrucci MT, Ricciardi MR, Gregorj C, Ciapponi L, Savino R, Ciliberto G, Tafuri A
    Leuk. Lymphoma, 2002;43(12):2369-75.
    Species: Human
    Sample Type: Serum
  30. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
    Authors: Carstanjen D, Regenfus M, Muller C, Salama A
    Exp. Hematol., 2001;29(7):812-21.
    Species: Human
    Sample Type: Plasma
  31. Ovariectomy does not induce osteopenia through interleukin-6 in rhesus monkeys (Macaca mulatta).
    Authors: Keller ET, 49536, Binkley NC, Stebler BA, Hall DM, Johnston GM, Zhang J, Ershler WB
    Bone, 2000;26(1):55-62.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Bone Marrow Fluid
  32. Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130.
    Authors: Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H
    Neurosci. Lett., 1999;259(3):145-8.
    Species: Human
    Sample Type: CSF
  33. Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease.
    Authors: Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ
    Brain Res., 1999;823(1):104-12.
    Species: Human
    Sample Type: CSF
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