Human IL-8/CXCL8 Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 beta.
  • Source
    Monoclonal Mouse IgG1 Clone # 6217
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CXCL8/IL‑8
    Ser28-Ser99
    Accession # P10145
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL‑8 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A.Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-8/CXCL8
CXCL8/IL-8 is an 8-9 kDa member of the CXCL subfamily of chemokines. It was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It has also referred to as Neutrophil Activating Protein (NAP), Monocyte-derived Neutrophil Chemotactic Factor (MDNCF) and granulocyte chemotactic protein (GCP). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and mammary plus alveolar epithelium can produce CXCL8 in response to a wide variety of stimuli, such as IL-1b, TNF-a, LPS, MRP-8/14 and viruses. When secreted, it circulates as both a monomer and dimer, with the dimeric forms being a homodimer and heterodimer with CXCL4/PF4. CXCL8 binds to CXCR-1 and -2, with the CXCL8 monomer favoring CXCR-1, and the CXCL8 homodimer favoring CXCR-2 and glycosaminoglycans. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. It also induces expression of the cell adhesion molecules CD11/CD18, and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes, and has also shown to be an autocrine growth factor for melanoma cells. CXCL8 is also reported to be angiogenic both in vivo and in vitro. There is no direct structural rodent counterpart to human IL-8.
  • Long Name:
    Interleukin 8
  • Entrez Gene IDs:
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
  • Alternate Names:
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011;6(1):e15997.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
    Authors: Mendez-Cruz AR, Paez A, Jimenez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, Lopez-Marure R, Masso FA, Flores-Romo L, Montano LF
    Immunol. Lett., 2007;111(2):116-23.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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