|Detection of Human Importin alpha 2/KPNA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human Importin alpha 2/KPNA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Importin alpha 2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line. Importin alpha 2/KPNA2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Importin alpha 2/KPNA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
KPNA2 (Karyopherin subunit alpha 2; also SRP1 alpha, importin subunit alpha 2 and RAG1) is a 58-60 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, KPNA2 first binds to NBS1, and then to importin beta 1, which mediates movement through the nuclear pore. Human KPNA2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 2-60), ten Armadillo repeats that bind "cargo" (aa 71-496) and three intervening NLSs. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human KPNA2 shares 94% aa identity with mouse KPNA2.