Human IRAK4 Antibody

Catalog # Availability Size / Price Qty
MAB3919-100
MAB3919-SP
IRAK4 in Human Tonsil.
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Human IRAK4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRAK4 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 413609
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IRAK4 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IRAK4 in Human Tonsil. IRAK4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Human IRAK4 Monoclonal Antibody (Catalog # MAB3919) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRAK4

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Long Name
Interleukin-1 Receptor-Associated Kinase 4
Entrez Gene IDs
51135 (Human); 266632 (Mouse); 300177 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; interleukin-1 receptor-associated kinase 4; IPD1; IRAK4; IRAK-4; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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