Human IRS1 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB39781R Datasheet / COA / SDS

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FAB39781R-100UG

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Human IRS1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse IRS1 in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 395601

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human IRS1
Ala801-Ala1020 (predicted)
Accession # P35568

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.

Long Name

Insulin Receptor Substrate 1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Alternate Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1