IRS1 in MCF‑7 Human Cell Line. IRS1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human IRS1 Monoclonal Antibody (Catalog # MAB39781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate
intermediate between the insulin and IGF-I receptor, and downstream
signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is
tyrosine phosphorylated, allowing its association with PI-3 kinase and
GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH
(pleckstrin homology) domain (aa 12-115), followed by a PTB
(phosphotyrosine-binding) domain (aa 160-263) and ten PEST
(Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically
cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Over
aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse
and pig IRS1, respectively.
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