Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Islet-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Islet-2
Met1-Thr359
Accession # Q96A47

Specificity

Detects human Islet-2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Islet‑2 Antibody

Detection of Human Islet‑2 antibody by Western Blot.

Detection of Human Islet‑2 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human Islet-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Islet-2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Islet‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Islet-2

Islet-2 is a member of the LIM homeodomain transcription factors and its expression pattern defines a subclass of motor neurons. Islet-2 contains two amino terminal LIM domains and a carboxy terminal homeodomain. Human Islet-2 shares 93% similarity with mouse Islet-2.

Alternate Names

ISL2, Islet2

Entrez Gene IDs

64843 (Human); 104360 (Mouse); 57233 (Rat)

Gene Symbol

ISL2

UniProt

Q96A47

Additional Islet-2 Products

Product Documents for Human Islet‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Islet‑2 Antibody

For research use only

Citations for Human Islet‑2 Antibody

Customer Reviews for Human Islet‑2 Antibody

There are currently no reviews for this product. Be the first to review Human Islet‑2 Antibody and earn rewards!

Have you used Human Islet‑2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies