Human Islet-2 Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human Islet‑2 by Western Blot.
1 Image
Product Details
Supplemental Products

Human Islet-2 Antibody Summary

Species Reactivity
Detects human Islet-2 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Islet-2
Accession # Q96A47
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Islet‑2 antibody by Western Blot. View Larger

Detection of Human Islet‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human Islet-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Islet-2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Islet-2

Islet-2 is a member of the LIM homeodomain transcription factors and its expression pattern defines a subclass of motor neurons. Islet-2 contains two amino terminal LIM domains and a carboxy terminal homeodomain. Human Islet-2 shares 93% similarity with mouse Islet-2.

Entrez Gene IDs
64843 (Human); 104360 (Mouse); 57233 (Rat)
Alternate Names
FLJ10160; insulin gene enhancer protein ISL-2; ISL LIM homeobox 2; ISL2 transcription factor, LIM/homeodomain, (islet-2); ISL2; Islet2; Islet-2

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Islet-2 Antibody

There are currently no reviews for this product. Be the first to review Human Islet-2 Antibody and earn rewards!

Have you used Human Islet-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review