Human Jagged 1 Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat Jagged 1 is observed and less than 1% cross-reactivity with recombinant human Jagged 2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Jagged 1
    Ser32-Asp296
    Accession # P78504
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

  • References:
    1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
    2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
    3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
    4. Lai, E.C. (2004) Development 131:965.
    5. Milner, L.A. et al. (1994) Blood 83:2057.  
    6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
    7. Linheng, L. et al. (1998) Immunity 8:43.
    8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
    9. Oda, T. et al. (1997) Nat. Genet. 16:235.
    10. Oda, T. et al. (1997) Genomics 43:376.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Applications
Sample Type
  1. Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles
    Authors: P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015;75(15):3098-107.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2012;72(1):66-75.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Interleukin-6 as a therapeutic target in human ovarian cancer.
    Authors: Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
    Clin. Cancer Res., 2011;17(18):6083-96.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
    Authors: Roesli C, Mumprecht V, Neri D, Detmar M
    FASEB J., 2008;22(6):1933-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007;26(1):279-89.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Jagged 1 Antibody AF1277
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 ICC/IF Human Anonymous 12/10/2017
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Immunocytochemistry/Immunofluorescence Human Jagged 1 Antibody AF1277
Immunocytochemistry/Immunofluorescence: Human Jagged 1 Antibody [AF1277]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedRT4-D6P2T rat schwannoma cell line
SpeciesHuman

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