Human Jagged 1 Antibody

Catalog # Availability Size / Price Qty
AF1277
AF1277-SP
Human Jagged 1 Antibody in Western Blot
3 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human Jagged 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant rat Jagged 1 and recombinant human Jagged 2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Jagged 1 by Western Blot. View Larger

Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Jagged 1 in Human Kidney Cancer Tissue. View Larger

Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Neutralization Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. View Larger

Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Product Datasheets

Citations for Human Jagged 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells
    Authors: M Bellon, R Moles, H Chaib-Mezr, J Pancewicz, C Nicot
    J Hematol Oncol, 2018;11(1):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation
    Authors: F De Falco, B Del Papa, S Baldoni, R Sabatini, F Falzetti, M Di Ianni, MP Martelli, F Mezzasoma, M Pelullo, P Marconi, P Sportolett, I Screpanti, E Rosati
    Cell Death Dis, 2018;9(12):1160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles
    Authors: P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015;75(15):3098-107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2012;72(1):66-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Interleukin-6 as a therapeutic target in human ovarian cancer.
    Authors: Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
    Clin. Cancer Res., 2011;17(18):6083-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
    Authors: Roesli C, Mumprecht V, Neri D, Detmar M
    FASEB J., 2008;22(6):1933-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007;26(1):279-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human Jagged 1 Antibody
By Anonymous on 12/10/2017
Application: ICC/IF Sample Tested: RT4-D6P2T rat schwannoma cell line Species: Human