Human Jagged 1 Antibody

R&D Systems | Catalog # AF1277

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, In vivo assay, In-Cell Western (ICW)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Jagged 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504

Specificity

Detects human Jagged 1 in direct ELISAs. In direct ELISA less than 25% cross-reactivity with recombinant rat Jagged 1 and recombinant human Jagged 2 is observed. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Jagged 1 Antibody

Detection of Human Jagged 1 antibody by Western Blot.

Detection of Human Jagged 1 by Western Blot.

Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Jagged 1 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

Jagged 1 in Human Kidney Cancer Tissue.

Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody.

Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody.

Recombinant Human Jagged 1 Fc Chimera (1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.
Detection of Jagged 1 by Immunohistochemistry

Detection of Jagged 1 by Immunohistochemistry

Correlation analysis of the Jagged ligands, Notch activation and angiogenesis in MM patients’ BMBs. (A) representative images of the antigen immunoreactivity for the monoclonal Ig light chain, Jagged1, Jagged2, HES6, VEGF-A and CD34 in BMBs from 34 MM patients at different degrees of tumor infiltration (I: less than 20%; II: 21–50%; III: >51%). The arrows indicate specific immunoreactive signals. Photos were acquired at Nano-Zoomer 2.0 and scale bar is for 250 µm. Pearson’s correlation coefficient (r) and the p-values are reported for the correlation analyses between (B) Jagged1 and Jagged 2 expressed in MM cells and the Notch transcriptional target HES6 in the same tumor cells; (C) HES6 and VEGF-A expressed in MM cells or MVD; (D) Jagged1 and Jagged 2 expressed in MM cells and the Notch transcriptional target HES6 in nearby non-tumor cells; and (E) HES6 in non-tumor cells and VEGF-A in non-tumor cells and MVD. M = MM cells; NM = non tumor cells. Statistical analysis was carried out using two-tailed t-tests. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32932949), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Jagged 1 by Western Blot

Detection of Jagged 1 by Western Blot

Multiple myeloma (MM) cell-derived Jagged promotes angiogenesis: (A) Up: Jagged1 and 2 knock-down (KD) efficiency in RPMI8226KD and OPM2KD cells was obtained by qRT-PCR assay of the relative gene expression variation (normalized to GAPDH) of Jagged1 and Jagged2 and the Notch target gene HES6, calculated by the 2− delta delta Ct formula. Data are expressed as the mean value ± SD. Down: Western blot analysis of Jagged1 and Jagged2 in HMCLsSCR or HCMLsKD. Protein loading was normalized to -actin. The shown images are representative of three independent experiments. (B) Up: Tube formation assay on Matrigel with co-culture systems of HMCLsSCR or HCMLsKD and primary human pulmonary artery endothelial cells (HPAECs). 4X magnification images are shown. Down: Graphs show quantification of the number of areas and branch points and total tube length. (C) HPAEC tube formation stimulated by Jagged1 and Jagged2 peptides. Images are a 4X magnification. Statistical analysis was carried out by a one-tailed t test; * is for p ≤ 0.05; ** is for p ≤ 0.01; *** is for p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32932949), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Jagged 1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Western Blot

1 µg/mL
Sample: Huh‑7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line

Neutralization

Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.

Reviewed Applications

Read 2 reviews rated 4.5 using AF1277 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

Alternate Names

CD339, JAG1

Entrez Gene IDs

182 (Human); 16449 (Mouse); 29146 (Rat)

Gene Symbol

JAG1

UniProt

P78504 (Human)

Additional Jagged 1 Products

Product Documents for Human Jagged 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Jagged 1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Notch Pathway

Citations for Human Jagged 1 Antibody

Customer Reviews for Human Jagged 1 Antibody (2)

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2 Customer Ratings
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  • Human Jagged 1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 11/12/2025
    Human Jagged 1 Antibody AF1277
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human Jagged 1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: RT4-D6P2T rat schwannoma cell line
    Species: Human
    Verified Customer | Posted 12/10/2017
    Human Jagged 1 Antibody AF1277

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