Human LAMP Antibody

Catalog # Availability Size / Price Qty
MAB8731
MAB8731-SP
Product Details
FAQs
Supplemental Products
Reviews

Human LAMP Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAMP in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 110722
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP
Val29-Asn315
Accession # AAC50569
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize the the enhancemment of neurite outgrowth of cortical neurons from E16-E18 rat embryos induced by LAMP. The Neutralization Dose (ND50) is typically 15 µg/mL in the presence of 10 µg/mL Recombinant Human LAMP Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP

LAMP (limbic system-associated membrane protein) is a member of the IgLON (immunoglobulin LAMP, OBCAM and neurotrimin) subfamily within the Ig superfamily. All IgLON family members are glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecules that are involved in cell-cell recognition and may have a role in mediating selective neuronal growth and axon targeting. LAMP cDNA encodes a 338 amino acid (aa) residues precursor protein containing a 28 aa N-terminal signal peptide, a 23 aa C-terminal propeptide and a 287 aa mature chain with 3 Ig-like C2-type domains and a GPI-anchor attachment site. In the developing brain, LAMP exhibits a specific pattern of expression in the cortical and subcortical limbic areas, which are important in cognition, emotion, memory, and learning. LAMP is also expressed in single layers of the superior colliculus, spinal chord and cerebellum. LAMP promotes adhesion and growth of limbic axons primarily via homophilic interaction and in part by modulating calcium influx through L-type calcium channels in limbic neurons. Heterophilic interactions between LAMP and neurotrimin have also been demonstrated. LAMP has been shown to inhibit the outgrowth of neurotrimin-expressing dorsal root ganglion neurons in a heterophilic manner. Antibody perturbation studies showed that LAMP is necessary for normal circuit formation in the limbic system, including the septo-hippocampal connection and hippocampal mossy fibers. LAMP acts as an attractive guidance signal for the limbic thalamic axons and can induce branch formation, but also acts as a repulsive axon guidance signal for nonlimbic thalamic axons (1‑5).

References
  1. Pimenta, A.F. et al. (1995) Neuron, 15:287.
  2. Zhukareva, V. and P. Levitt (1995) Development 121:1161.
  3. Zhukareva, V. et al. (1997) Mol. Cell Neurosci. 10:43.
  4. Mann, F. et al. (1998) Journal Neurosci. 18:9409.
  5. Gil, O.D. et al. (2002) J. Neurobiol. 51:190.
Long Name
Limbic System-associated Membrane Protein
Entrez Gene IDs
4045 (Human)
Alternate Names
FLJ34254; FLJ35396; IgLON family member 3; IGLON3FLJ54658; LAMP; LAMPFLJ37216; limbic system-associated membrane protein; LSAMP

Product Datasheets

Product Specific Notices

US Patents # 5,861,283; 6,423,827 and patents pending.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human LAMP Antibody

There are currently no reviews for this product. Be the first to review Human LAMP Antibody and earn rewards!

Have you used Human LAMP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review