Human Latent TGF-beta  bp1 Antibody

Catalog # Availability Size / Price Qty
MAB388-SP
MAB388-500
MAB388-100
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (3)

Human Latent TGF-beta  bp1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LTBP-1 from the large latent TGF-beta 1 complex.
Source
Monoclonal Mouse IgG1 Clone # 35409
Purification
Protein A or G purified from ascites
Immunogen
Human platelet-derived large latent TGF-beta 1 complex
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Large latent TGF-beta complex from human platelets
under non-reducing conditions only
Immunoaffinity Purification
This antibody can be used to affinity purify the large latent TGF-beta complex (containing both the 230 and 195 kDa binding protein) and will also pull out “free” binding protein that is not associated with the small latent TGF-beta.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latent TGF-beta bp1

TGF-beta  is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-beta 1 complex is composed of three components: mature TGF-beta 1 dimer; latency associated peptide; and a latent TGF-beta binding protein (LTBP) (1-3).The cDNAs for four binding proteins (LTBP-1, -2, -3 and -4) have now been cloned (4-5).

References
  1. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  2. Moren, A. et al. (1994) J. Biol. Chem. 269:32469.
  3. Yin, W. et al. (1995) J. Biol. Chem. 270:10147.
  4. Kanzaki, T. et al. (1990) Cell 61:1051.
  5. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.
Entrez Gene IDs
4052 (Human)
Alternate Names
Latent TGFbeta bp1; Latent TGF-beta bp1; latent transforming growth factor beta binding protein 1; latent-transforming growth factor beta-binding protein 1; LTBP1; LTBP-1; MGC163161; TGF-beta1-BP-1; Transforming growth factor beta-1-binding protein 1

Product Datasheets

Citations for Human Latent TGF-beta  bp1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018;8(1):603.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. ATRA modulates mechanical activation of TGF-? by pancreatic stellate cells
    Sci Rep, 2016;6(0):27639.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Identification of the thiol isomerase-binding peptide, mastoparan, as a novel inhibitor of shear-induced transforming growth factor beta1 (TGF-beta1) activation.
    Authors: Brophy T, Coller B, Ahamed J
    J Biol Chem, 2013;288(15):10628-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Kidney transplantation: analysis of the expression and T cell-mediated activation of latent TGF-beta.
    Authors: Willet J, Pichitsiri W, Jenkinson S, Brain J, Wood K, Alhasan A, Spielhofer J, Robertson H, Ali S, Kirby J
    J Leukoc Biol, 2013;93(4):471-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
    Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
    Exp. Cell Res., 2011;317(7):976-93.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
    Authors: Massam-Wu T, Chiu M, Choudhury R
    J. Cell. Sci., 2010;123(0):3006-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
    Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
    J. Pathol., 2009;218(1):131-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Matrix association of latent TGF-beta binding protein-2 (LTBP-2) is dependent on fibrillin-1.
    Authors: Vehvilainen P, Hyytiainen M, Keski-Oja J
    J. Cell. Physiol., 2009;221(3):586-93.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human Latent TGF-beta  bp1 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human Latent TGF-beta  bp1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Latent TGF-beta  bp1 Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Latent TGF-beta  bp1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human