Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, Immunoaffinity Purification

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 35409
View all Latent TGF-beta bp1 Primary Antibodies »

Product Specifications

Immunogen

Human platelet-derived large latent TGF-beta 1 complex

Specificity

Detects human LTBP-1 from the large latent TGF-beta 1 complex.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Latent TGF‑ beta  bp1 Antibody

Detection of Latent TGF¿ beta bp1 by Western Blot

Detection of Latent TGF¿ beta bp1 by Western Blot

LTBP-1 accumulates in CADASIL brain vessels and co-localizes with Notch3-ECD aggregates. a Frozen brain sections from a CADASIL patient and a healthy control containing an arteriole (left panels) or a capillary (right panels) were stained for LTBP-1 and analyzed by fluorescence microscopy. b Frozen brain sections of three CADASIL patients were stained for LTBP-1 and Notch3-ECD and analyzed by confocal fluorescence microscopy. c LTBP-1 co-fractionates with Notch3-ECD. The final ( beta -ME-containing) fractions of sequentially extracted brain vessels from CADASIL patients and controls were immunoblotted for Notch3-ECD, LTBP-1 and beta -tubulin. Shown is a representative blot from two independent experiments. (CAD: CADASIL patient; Ctrl: control). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25190493), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Latent TGF‑ beta  bp1 Antibody

Application
Recommended Usage

Immunoaffinity Purification

This antibody can be used to affinity purify the large latent TGF-beta complex (containing both the 230 and 195 kDa binding protein) and will also pull out “free” binding protein that is not associated with the small latent TGF-beta.

Western Blot

1 µg/mL
Sample: Large latent TGF-beta complex from human platelets
under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 3.7 using MAB388 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Latent TGF-beta bp1

TGF-beta  is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-beta 1 complex is composed of three components: mature TGF-beta 1 dimer; latency associated peptide; and a latent TGF-beta binding protein (LTBP) (1-3).The cDNAs for four binding proteins (LTBP-1, -2, -3 and -4) have now been cloned (4-5).

References

  1. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  2. Moren, A. et al. (1994) J. Biol. Chem. 269:32469.
  3. Yin, W. et al. (1995) J. Biol. Chem. 270:10147.
  4. Kanzaki, T. et al. (1990) Cell 61:1051.
  5. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.

Alternate Names

Latent TGFbeta bp1, LTBP1

Entrez Gene IDs

4052 (Human)

Gene Symbol

LTBP1

Additional Latent TGF-beta bp1 Products

Product Documents for Human Latent TGF‑ beta  bp1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Latent TGF‑ beta  bp1 Antibody

For research use only

Citations for Human Latent TGF‑ beta  bp1 Antibody

Customer Reviews for Human Latent TGF‑ beta  bp1 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Latent TGF‑ beta  bp1 Antibody MAB388
  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Latent TGF‑ beta  bp1 Antibody MAB388
  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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