Human Latent TGF-beta bp1 Antibody (35409)

R&D Systems | Catalog # MAB388

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, Immunoaffinity Purification

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 35409
View all Latent TGF-beta bp1 Primary Antibodies »

Product Specifications

Immunogen

Human platelet-derived large latent TGF-beta 1 complex

Specificity

Detects human LTBP-1 from the large latent TGF-beta 1 complex.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Latent TGF-beta bp1 Antibody (35409)

Detection of Latent TGF¿ beta bp1 by Western Blot

Detection of Latent TGF¿ beta bp1 by Western Blot

LTBP-1 accumulates in CADASIL brain vessels and co-localizes with Notch3-ECD aggregates. a Frozen brain sections from a CADASIL patient and a healthy control containing an arteriole (left panels) or a capillary (right panels) were stained for LTBP-1 and analyzed by fluorescence microscopy. b Frozen brain sections of three CADASIL patients were stained for LTBP-1 and Notch3-ECD and analyzed by confocal fluorescence microscopy. c LTBP-1 co-fractionates with Notch3-ECD. The final ( beta -ME-containing) fractions of sequentially extracted brain vessels from CADASIL patients and controls were immunoblotted for Notch3-ECD, LTBP-1 and beta -tubulin. Shown is a representative blot from two independent experiments. (CAD: CADASIL patient; Ctrl: control). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25190493), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Latent TGF-beta bp1 Antibody (35409)

Application
Recommended Usage

Immunoaffinity Purification

This antibody can be used to affinity purify the large latent TGF-beta complex (containing both the 230 and 195 kDa binding protein) and will also pull out “free” binding protein that is not associated with the small latent TGF-beta.

Western Blot

1 µg/mL
Sample: Large latent TGF-beta complex from human platelets
under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 3.7 using MAB388 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Latent TGF-beta bp1

TGF-beta  is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-beta 1 complex is composed of three components: mature TGF-beta 1 dimer; latency associated peptide; and a latent TGF-beta binding protein (LTBP) (1-3).The cDNAs for four binding proteins (LTBP-1, -2, -3 and -4) have now been cloned (4-5).

References

  1. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  2. Moren, A. et al. (1994) J. Biol. Chem. 269:32469.
  3. Yin, W. et al. (1995) J. Biol. Chem. 270:10147.
  4. Kanzaki, T. et al. (1990) Cell 61:1051.
  5. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.

Alternate Names

Latent TGFbeta bp1, LTBP1

Entrez Gene IDs

4052 (Human)

Gene Symbol

LTBP1

Additional Latent TGF-beta bp1 Products

Product Documents for Human Latent TGF-beta bp1 Antibody (35409)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Latent TGF-beta bp1 Antibody (35409)

For research use only

Citations for Human Latent TGF-beta bp1 Antibody (35409)

Customer Reviews for Human Latent TGF-beta bp1 Antibody (35409) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Latent TGF-beta bp1 Antibody (35409) MAB388
  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Latent TGF-beta bp1 Antibody (35409) MAB388
  • Human Latent TGF-beta  bp1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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