Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human SPINK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8515) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SPINK5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LEKTI (Lympho-epithelial Kazal-type-related inhibitor; also SPINK5, Serine Protease Inhibitor Kazal-type 5) is a 150 kDa precursor protein that is cleaved into the following 2 chains: Hemofiltrate peptide HF6478 and Hemofiltrate peptide HF7665. LEKTI is a multidomain serine protease inhibitor that contains 15 Kazal-like inhibitory domains. It may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. It contains fifteen Kazal-like domains. Mature human SPINK5 shares 60% aa sequence identity with mouse SPINK5.
Serine Peptidase Inhibitor, Kazal Type 5
Entrez Gene IDs:
11005 (Human); 72432 (Mouse); 361319 (Rat)
Kazal type 5; LEKTI; LETKI; NETS; serine peptidase inhibitor, Kazal type 5; SPINK5; VAKTI
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