Human Leptin Quantikine ELISA Kit

  (67 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (67)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    7.8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human Leptin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC237 , Quantikine Immunoassay Control Group 237 - Please Inquire
Product Summary
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural human Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 64.5 146 621 65.7 146 581
Standard Deviation 2.14 4.32 20 3.56 6.17 20.6
CV% 3.3 3 3.2 5.4 4.2 3.5

Recovery

The recovery of Leptin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 94-102
Citrate Plasma (n=5) 95 87-105
EDTA Plasma (n=5) 99 85-112
Heparin Plasma (n=5) 90 81-100
Serum (n=5) 95 89-109
Linearity
To assess the linearity of the assay, cell culture media samples spiked with high concentrations of Leptin and natural serum/plasma samples were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Leptin/OB [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin/OB
Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.
  • Entrez Gene IDs:
    3952 (Human); 16846 (Mouse); 25608 (Rat)
  • Alternate Names:
    FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

67 Citations: Showing 1 - 10
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Species
Sample Type
  1. Plasma leptin, but not resistin, TNF-? and adiponectin, is associated with echocardiographic parameters of cardiac remodeling in patients with coronary artery disease
    Authors: AD Farca?, A Rusu, MA Stoia, LA Vida-Simit
    Cytokine, 2018;103(0):46-49.
    Species: Human
    Sample Type: Plasma
  2. Relationships of insulin-like growth factor-1, its binding proteins, and cardiometabolic risk in hypertensive perimenopausal women
    Authors: A Olszanecka, A Dragan, K Kawecka-Ja, D Fedak, D Czarnecka
    Metab. Clin. Exp., 2017;69(0):96-106.
    Species: Human
    Sample Type: Plasma
  3. Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial
    Authors: TH Kim, JS Chang, KS Park, J Park, N Kim, JI Lee, ID Kong
    PLoS ONE, 2017;12(2):e0171771.
    Species: Human
    Sample Type: Serum
  4. Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood
    Authors: DY Joh, AM Hucknall, Q Wei, KA Mason, ML Lund, CM Fontes, RT Hill, R Blair, Z Zimmers, RK Achar, D Tseng, R Gordan, M Freemark, A Ozcan, A Chilkoti
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Serum
  5. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
    Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
    Dis. Markers, 2017;2017(0):8248175.
    Species: Human
    Sample Type: Tissue Homogenates
  6. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
    Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
    Oxid Med Cell Longev, 2017;2017(0):3937842.
    Species: Human
    Sample Type: Plasma
  7. Adipokines, C-reactive protein and Amyotrophic Lateral Sclerosis - results from a population- based ALS registry in Germany
    Authors: G Nagel, RS Peter, A Rosenbohm, W Koenig, L Dupuis, D Rothenbach, AC Ludolph
    Sci Rep, 2017;7(1):4374.
    Species: Human
    Sample Type: Serum
  8. Serum levels of adiponectin and leptin as biomarkers of proteinuria in lupus nephritis
    Authors: V Diaz-Rizo, D Bonilla-La, L Gonzalez-L, D Sanchez-Mo, NS Fajardo-Ro, EE Perez-Guer, NA Rodriguez-, AM Saldaña-Cr, ML Vazquez-Vi, E Gomez-Bañu, M Vazquez-De, EG Cardona-Mu, D Cardona-Mu, X Trujillo, M Huerta, M Salazar-Pa, JI Gamez-Nava
    PLoS ONE, 2017;12(9):e0184056.
    Species: Human
    Sample Type: Serum
  9. Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels
    Authors: KS Rana, C Pararasa, I Afzal, DA Nagel, EJ Hill, CJ Bailey, HR Griffiths, I Kyrou, HS Randeva, S Bellary, JE Brown
    Cardiovasc Diabetol, 2017;16(1):147.
    Species: Human
    Sample Type: Plasma
  10. Key Clinical Factors Predicting Adipokine and Oxidative Stress Marker Concentrations among Normal, Overweight and Obese Pregnant Women Using Artificial Neural Networks
    Authors: M Solis-Pare, G Estrada-Gu, O Perichart-, A Montoya-Es, M Guzmán-Hue, H Borboa-Oli, E Bravo-Flor, A Cardona-Pé, V Zaga-Clave, E Garcia-Lat, G Gonzalez-P, JA Hernández-, C Irles
    Int J Mol Sci, 2017;19(1):.
    Species: Human
    Sample Type: Serum
  11. Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults
    Authors: LP Carvalho, RP Basso-Vane, L Di Thommaz, RG Mendes, MC Oliveira-J, RP Vieira, JC Bonjorno-J, CR Oliveira, R Luporini, A Borghi-Sil
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  12. Endurance exercise training decreased serum levels of surfactant protein D and improved aerobic fitness of obese women with type-2 diabetes
    Authors: S Rezaei, MM Shamsi, M Mahdavi, A Jamali, J Prestes, RA Tibana, JW Navalta, FA Voltarelli
    Diabetol Metab Syndr, 2017;9(0):74.
    Species: Human
    Sample Type: Serum
  13. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2017;4(1):56-65.
    Species: Human
    Sample Type: Serum
  14. Serum levels of leptin and adiponectin and clinical parameters in women with fibromyalgia and overweight/obesity
    Authors: ES Paiva, A Andretta, ED Batista, MM Lobo, RC Miranda, R Nisihara, ME Schieferde, CL Boguszewsk
    Arch Endocrinol Metab, 2017;0(0):0.
    Species: Human
    Sample Type: Serum
  15. The effect of BMI, serum leptin, and adiponectin levels on prognosis in patients with non-ischaemic dilated cardiomyopathy
    Authors: C Wojciechow, W Jache?, E Romuk, E Nowalany-K, A Tomasik, L Siemi?ska
    Endokrynol Pol, 2017;68(1):26-34.
    Species: Human
    Sample Type: Serum
  16. Impact of physical activity level and dietary fat content on passive overconsumption of energy in non-obese adults
    Authors: K Beaulieu, M Hopkins, J Blundell, G Finlayson
    Int J Behav Nutr Phys Act, 2017;14(1):14.
    Species: Human
    Sample Type: Plasma
  17. Altered Systemic Adipokines in Patients with Chronic Urticaria
    Authors: Hoang Kim Tu Trinh
    Int. Arch. Allergy Immunol, 2016;171(2):102-110.
    Species: Human
    Sample Type: Serum
  18. Maternal obesity drives functional alterations in uterine NK cells
    JCI Insight, 2016;1(11):e85560.
    Species: Human
    Sample Type: Serum
  19. Metabolic Syndrome and Selective Inflammatory Markers in Psoriatic Patients
    Authors: S Vachatova, C Andrys, J Krejsek, M Salavec, K Ettler, V Rehacek, E Cermakova, A Malkova, Z Fiala, L Borska
    J Immunol Res, 2016;2016(0):5380792.
    Species: Human
    Sample Type: Serum
  20. Dietary patterns and cardiometabolic and endocrine plasma biomarkers in US women
    Authors: Hala B AlEssa
    Am. J. Clin. Nutr, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  21. Leptin, Leptin Soluble Receptor, and the Free Leptin Index following a Diet and Physical Activity Lifestyle Intervention in Obese Males and Females
    Authors: Jeffrey E Herrick
    J Obes, 2016;2016(0):8375828.
    Species: Human
    Sample Type: Serum
  22. Effects of different degrees of insulin resistance on endothelial function in�obese�adults undergoing alternate day�fasting
    Authors: Kristin K Hoddy
    Nutr Healthy Aging, 2016;4(1):63-71.
    Species: Human
    Sample Type: Plasma
  23. Type 3 Deiodinase Role on Central Thyroid Hormone Action Affects the Leptin-Melanocortin System and Circadian Activity
    Authors: Zhaofei Wu
    Endocrinology, 2016;0(0):en20161680.
    Species: Human
    Sample Type: Serum
  24. Leptin levels are negatively correlated with 2-arachidonoylglycerol in the cerebrospinal fluid of patients with osteoarthritis.
    Authors: Nicholson J, Azim S, Rebecchi M, Galbavy W, Feng T, Reinsel R, Rizwan S, Fowler C, Benveniste H, Kaczocha M
    PLoS ONE, 2015;10(4):e0123132.
    Species: Human
    Sample Type: Serum
  25. Additive regulation of adiponectin expression by the mediterranean diet olive oil components oleic Acid and hydroxytyrosol in human adipocytes.
    Authors: Scoditti E, Massaro M, Carluccio M, Pellegrino M, Wabitsch M, Calabriso N, Storelli C, De Caterina R
    PLoS ONE, 2015;10(6):e0128218.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
    Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
    BMC Cancer, 2015;15(0):492.
    Species: Human
    Sample Type: Peritoneal Fluid
  27. Lower Circulating C1q/TNF-Related Protein-3 (CTRP3) Levels Are Associated with Obesity: A Cross-Sectional Study.
    Authors: Wolf R, Steele K, Peterson L, Magnuson T, Schweitzer M, Wong G
    PLoS ONE, 2015;10(7):e0133955.
    Species: Human
    Sample Type: Serum
  28. A cross-over experiment to investigate possible mechanisms for lower BMIs in people who habitually eat breakfast.
    Authors: Reeves S, Huber J, Halsey L, Villegas-Montes M, Elgumati J, Smith T
    Eur J Clin Nutr, 2015;69(5):632-7.
    Species: Human
    Sample Type: Serum
  29. Cord blood adipokines, neonatal anthropometrics and postnatal growth in offspring of Hispanic and Native American women with diabetes mellitus.
    Authors: Teague A, Fields D, Aston C, Short K, Lyons T, Chernausek S
    Reprod Biol Endocrinol, 2015;13(0):68.
    Species: Human
    Sample Type: Serum
  30. A Dietary Supplement Containing Cinnamon, Chromium and Carnosine Decreases Fasting Plasma Glucose and Increases Lean Mass in Overweight or Obese Pre-Diabetic Subjects: A Randomized, Placebo-Controlled Trial.
    Authors: Liu Y, Cotillard A, Vatier C, Bastard J, Fellahi S, Stevant M, Allatif O, Langlois C, Bieuvelet S, Brochot A, Guilbot A, Clement K, Rizkalla S
    PLoS ONE, 2015;10(9):e0138646.
    Species: Human
    Sample Type: Plasma
  31. Circulating adipokines and insulin resistance in subjects with combined cardiac and metabolic syndrome X.
    Authors: Liang K, Lee W, Lee W, Liao Y, Wang K, Lee I, Wang J, Sheu W
    Diabetol Metab Syndr, 2015;7(0):83.
    Species: Human
    Sample Type: Serum
  32. Adiponectin is a candidate biomarker of lower extremity bone density in men with chronic spinal cord injury.
    Authors: Doherty A, Battaglino R, Donovan J, Gagnon D, Lazzari A, Garshick E, Zafonte R, Morse L
    J Bone Miner Res, 2014;29(1):251-9.
    Species: Human
    Sample Type: Plasma
  33. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C.
    Authors: Mera K, Uto H, Mawatari S, Ido A, Yoshimine Y, Nosaki T, Oda K, Tabu K, Kumagai K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Hidaka M, Eguchi S, Tsubouchi H
    BMC Gastroenterol, 2014;14(0):27.
    Species: Human
    Sample Type: Serum
  34. Leptin is associated with vascular endothelial function in overweight patients with type 2 diabetes.
    Authors: Morioka T, Emoto M, Yamazaki Y, Kawano N, Imamura S, Numaguchi R, Urata H, Motoyama K, Mori K, Fukumoto S, Koyama H, Shoji T, Inaba M
    Cardiovasc Diabetol, 2014;13(0):10.
    Species: Human
    Sample Type: Plasma
  35. Regional adipose tissue hormone/cytokine production before and after weight loss in abdominally obese women.
    Authors: You T, Wang X, Murphy K, Lyles M, Demons J, Yang R, Gong D, Nicklas B
    Obesity (Silver Spring), 2014;22(7):1679-84.
    Species: Human
    Sample Type: Plasma
  36. Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants.
    Authors: Hohenadel M, Thearle M, Grice B, Huang H, Dai M, Tao Y, Hunter L, Palaguachi G, Mou Z, Kim R, Tsang M, Haack K, Voruganti V, Cole S, Butte N, Comuzzie A, Muller Y, Baier L, Krakoff J, Knowler W, Yanovski J, Han J
    Int J Obes (Lond), 2014;38(8):1068-74.
    Species: Human
    Sample Type: Serum
  37. Short-term intake of a Japanese-style healthy lunch menu contributes to prevention and/or improvement in metabolic syndrome among middle-aged men: a non-randomized controlled trial.
    Authors: Inoue H, Sasaki R, Aiso I, Kuwano T
    Lipids Health Dis, 2014;13(0):57.
    Species: Human
    Sample Type: Serum
  38. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013;8(12):e83042.
    Species: Human
    Sample Type: Plasma
  39. The type and quantity of dietary fat and carbohydrate alter faecal microbiome and short-chain fatty acid excretion in a metabolic syndrome 'at-risk' population.
    Authors: Fava, F, Gitau, R, Griffin, B A, Gibson, G R, Tuohy, K M, Lovegrove, J A
    Int J Obes (Lond), 2013;37(2):216-23.
    Species: Human
    Sample Type: Plasma
  40. A prospective study of circulating adipokine levels and risk of multiple myeloma.
    Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
    Blood, 2012;120(22):4418-20.
    Species: Human
    Sample Type: Plasma
  41. Alterations of leptin in the course of inflammation and severe sepsis.
    BMC Infect Dis, 2012;12(0):217.
    Species: Human
    Sample Type: Serum
  42. Adipocytokines and CD34 progenitor cells in Alzheimer's disease.
    Authors: Bigalke B, Schreitmuller B, Sopova K, Paul A, Stransky E, Gawaz M, Stellos K, Laske C
    PLoS ONE, 2011;6(5):e20286.
    Species: Human
    Sample Type: Plasma
  43. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
    Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
    Int J Obes (Lond), 2011;35(6):800-9.
    Species: Human
    Sample Type: Serum
  44. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
    Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
    Diabetes Care, 2010;33(8):1734-7.
    Species: Human
    Sample Type: Serum
  45. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
    Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
    Diabetes, 2010;59(12):3049-57.
    Species: Human
    Sample Type: Plasma
  46. Leptin exacerbates sepsis-mediated morbidity and mortality.
    Authors: Shapiro NI, Khankin EV, van Meurs M
    J. Immunol., 2010;185(1):517-24.
    Species: Human
    Sample Type: Plasma
  47. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
    Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
    Obesity (Silver Spring), 2010;18(11):2152-9.
    Species: Human
    Sample Type: Plasma
  48. Environmental tobacco smoke and cardiometabolic risk in young children: results from a survey in south-west Germany.
    Authors: Nagel G, Arnold FJ, Wilhelm M, Link B, Zoellner I, Koenig W
    Eur. Heart J., 2009;30(15):1885-93.
    Species: Human
    Sample Type: Plasma
  49. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
    Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
    AIDS, 2009;23(8):929-39.
    Species: Human
    Sample Type: Plasma
  50. Gene expression profiling of human skeletal muscle in response to stabilized weight loss.
    Authors: Larrouy D, Barbe P, Valle C, Dejean S, Pelloux V, Thalamas C, Bastard JP, Le Bouil A, Diquet B, Clement K, Langin D, Viguerie N
    Am. J. Clin. Nutr., 2008;88(1):125-32.
    Species: Human
    Sample Type: Plasma
  51. Diurnal variation of human sweet taste recognition thresholds is correlated with plasma leptin levels.
    Authors: Nakamura Y, Sanematsu K, Ohta R, Shirosaki S, Koyano K, Nonaka K, Shigemura N, Ninomiya Y
    Diabetes, 2008;57(10):2661-5.
    Species: Human
    Sample Type: Plasma
  52. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
    Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
    Clin. Chem., 2008;54(7):1176-82.
    Species: Human
    Sample Type: Serum
  53. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
    Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
    Thromb. Haemost., 2008;100(6):1123-9.
    Species: Human
    Sample Type: Plasma
  54. Positive energy balance is associated with accelerated muscle atrophy and increased erythrocyte glutathione turnover during 5 wk of bed rest.
    Authors: Biolo G, Agostini F, Simunic B, Sturma M, Torelli L, Preiser JC, Deby-Dupont G, Magni P, Strollo F, di Prampero P, Guarnieri G, Mekjavic IB, Pisot R, Narici MV
    Am. J. Clin. Nutr., 2008;88(4):950-8.
    Species: Human
    Sample Type: Plasma
  55. The association of gastric leptin with oesophageal inflammation and metaplasia.
    Authors: Francois F, Roper J, Goodman AJ, Pei Z, Ghumman M, Mourad M, de Perez AZ, Perez-Perez GI, Tseng CH, Blaser MJ
    Gut, 2007;57(1):16-24.
    Species: Human
    Sample Type: Serum
  56. Nitrosative stress predicts the presence and severity of nonalcoholic fatty liver at different stages of the development of insulin resistance and metabolic syndrome: possible role of vitamin A intake.
    Authors: Musso G, Gambino R, De Michieli F, Biroli G, Premoli A, Pagano G, Bo S, Durazzo M, Cassader M
    Am. J. Clin. Nutr., 2007;86(3):661-71.
    Species: Human
    Sample Type: Serum
  57. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
    Authors: Farese RV, Sajan MP, Yang H, Li P, Mastorides S, Gower WR, Nimal S, Choi CS, Kim S, Shulman GI, Kahn CR, Braun U, Leitges M
    J. Clin. Invest., 2007;117(8):2289-301.
    Species: Human
    Sample Type: Serum
  58. Panel of markers can accurately predict endometriosis in a subset of patients.
    Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
    Fertil. Steril., 2007;89(5):1073-81.
    Species: Human
    Sample Type: Serum
  59. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
    Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
    Fertil. Steril., 2007;90(1):60-4.
    Species: Human
    Sample Type: Follicular Fluid
  60. Obesity, adipokines, and abdominal aortic aneurysm: Health in Men study.
    Authors: Golledge J, Clancy P, Jamrozik K, Norman PE
    Circulation, 2007;116(20):2275-9.
    Species: Human
    Sample Type: Serum
  61. Plasma agouti-related protein levels in women with anorexia nervosa.
    Authors: Moriya J, Takimoto Y, Yoshiuchi K
    Psychoneuroendocrinology, 2006;31(9):1057-61.
    Species: Human
    Sample Type: Plasma
  62. Altered hepatic expression of SREBP-1 and PPARgamma is associated with liver injury in insulin-resistant lipodystrophic HIV-infected patients.
    Authors: Lemoine M, Barbu V, Girard PM, Kim M, Bastard JP, Wendum D, Paye F, Housset C, Capeau J, Serfaty L
    AIDS, 2006;20(3):387-95.
    Species: Human
    Sample Type: Serum
  63. Adiponectin and leptin in maternal serum, cord blood, and breast milk.
    Authors: Weyermann M, Beermann C, Brenner H, Rothenbacher D
    Clin. Chem., 2006;52(11):2095-102.
    Species: Human
    Sample Type: Serum
  64. Genetic regulation of the variation of circulating insulin-like growth factors and leptin in human pedigrees.
    Authors: Pantsulaia Ia, Pantsulaia I, Trofimov S, Kobyliansky E, Livshits G
    Metab. Clin. Exp., 2005;54(7):975-81.
    Species: Human
    Sample Type: Plasma
  65. Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.
    Authors: Chan DC, Watts GF, Ng TW, Uchida Y, Sakai N, Yamashita S, Barrett PH
    Clin. Chem., 2005;51(3):578-85.
    Species: Human
    Sample Type: Plasma
  66. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
    Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
    Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
    Species: Human
    Sample Type: Cell Culture Supernates
  67. Determinants of plasma leptin concentration in postmenopausal women.
    Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
    Mech. Ageing Dev., 2004;125(2):117-9.
    Species: Human
    Sample Type:
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Quantikine Immunoassay Control Group 237

Ctrl QC237  
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Average Rating: 5 (Based on 1 review)

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 Human Leptin Quantikine ELISA Kit DLP00 Array DLP00
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  Anonymous 05/15/2017
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 Human Leptin Quantikine ELISA Kit DLP00
: Human Leptin Quantikine ELISA Kit [DLP00].

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Sample Tested Human serum

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