Human Leptin Quantikine ELISA Kit

(51 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    7.8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human Leptin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC237, Quantikine Immunoassay Control Group 237 - Please Inquire
Product Summary
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural human Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean64.514662165.7146581
Standard Deviation2.144.32203.566.1720.6
CV%3.333.25.44.23.5

Recovery

The recovery of Leptin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 94-102
Citrate Plasma (n=5) 95 87-105
EDTA Plasma (n=5) 99 85-112
Heparin Plasma (n=5) 90 81-100
Serum (n=5) 95 89-109
Linearity
To assess the linearity of the assay, cell culture media samples spiked with high concentrations of Leptin and natural serum/plasma samples were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Leptin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin/OB
Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.
    • Entrez Gene IDs
      3952 (Human); 16846 (Mouse); 25608 (Rat);
    • Alternate Names
      FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    51 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Adipokines, C-reactive protein and Amyotrophic Lateral Sclerosis - results from a population- based ALS registry in Germany
      Authors: G Nagel, RS Peter, A Rosenbohm, W Koenig, L Dupuis, D Rothenbach, AC Ludolph
      Sci Rep, 2017;7(1):4374.
      Species: Human
      Sample Type: Serum
    2. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
      Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
      Oxid Med Cell Longev, 2017;2017(0):3937842.
      Species: Human
      Sample Type: Plasma
    3. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation
      Authors: D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P
      Dis. Markers, 2017;2017(0):8248175.
      Species: Human
      Sample Type: Tissue Homogenates
    4. Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood
      Authors: DY Joh, AM Hucknall, Q Wei, KA Mason, ML Lund, CM Fontes, RT Hill, R Blair, Z Zimmers, RK Achar, D Tseng, R Gordan, M Freemark, A Ozcan, A Chilkoti
      Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
      Species: Human
      Sample Type: Serum
    5. Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial
      Authors: TH Kim, JS Chang, KS Park, J Park, N Kim, JI Lee, ID Kong
      PLoS ONE, 2017;12(2):e0171771.
      Species: Human
      Sample Type: Serum
    6. Maternal obesity drives functional alterations in uterine NK cells
      JCI Insight, 2016;1(11):e85560.
      Species: Human
      Sample Type: Serum
    7. Metabolic Syndrome and Selective Inflammatory Markers in Psoriatic Patients
      Authors: S Vachatova, C Andrys, J Krejsek, M Salavec, K Ettler, V Rehacek, E Cermakova, A Malkova, Z Fiala, L Borska
      J Immunol Res, 2016;2016(0):5380792.
      Species: Human
      Sample Type: Serum
    8. Circulating adipokines and insulin resistance in subjects with combined cardiac and metabolic syndrome X.
      Authors: Liang K, Lee W, Lee W, Liao Y, Wang K, Lee I, Wang J, Sheu W
      Diabetol Metab Syndr, 2015;7(0):83.
      Species: Human
      Sample Type: Serum
    9. Cord blood adipokines, neonatal anthropometrics and postnatal growth in offspring of Hispanic and Native American women with diabetes mellitus.
      Authors: Teague A, Fields D, Aston C, Short K, Lyons T, Chernausek S
      Reprod Biol Endocrinol, 2015;13(0):68.
      Species: Human
      Sample Type: Serum
    10. A cross-over experiment to investigate possible mechanisms for lower BMIs in people who habitually eat breakfast.
      Authors: Reeves S, Huber J, Halsey L, Villegas-Montes M, Elgumati J, Smith T
      Eur J Clin Nutr, 2015;69(5):632-7.
      Species: Human
      Sample Type: Serum
    11. Lower Circulating C1q/TNF-Related Protein-3 (CTRP3) Levels Are Associated with Obesity: A Cross-Sectional Study.
      Authors: Wolf R, Steele K, Peterson L, Magnuson T, Schweitzer M, Wong G
      PLoS ONE, 2015;10(7):e0133955.
      Species: Human
      Sample Type: Serum
    12. A Dietary Supplement Containing Cinnamon, Chromium and Carnosine Decreases Fasting Plasma Glucose and Increases Lean Mass in Overweight or Obese Pre-Diabetic Subjects: A Randomized, Placebo-Controlled Trial.
      Authors: Liu Y, Cotillard A, Vatier C, Bastard J, Fellahi S, Stevant M, Allatif O, Langlois C, Bieuvelet S, Brochot A, Guilbot A, Clement K, Rizkalla S
      PLoS ONE, 2015;10(9):e0138646.
      Species: Human
      Sample Type: Plasma
    13. Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
      Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
      BMC Cancer, 2015;15(0):492.
      Species: Human
      Sample Type: Peritoneal Fluid
    14. Leptin levels are negatively correlated with 2-arachidonoylglycerol in the cerebrospinal fluid of patients with osteoarthritis.
      Authors: Nicholson J, Azim S, Rebecchi M, Galbavy W, Feng T, Reinsel R, Rizwan S, Fowler C, Benveniste H, Kaczocha M
      PLoS ONE, 2015;10(4):e0123132.
      Species: Human
      Sample Type: Serum
    15. Additive regulation of adiponectin expression by the mediterranean diet olive oil components oleic Acid and hydroxytyrosol in human adipocytes.
      Authors: Scoditti E, Massaro M, Carluccio M, Pellegrino M, Wabitsch M, Calabriso N, Storelli C, De Caterina R
      PLoS ONE, 2015;10(6):e0128218.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants.
      Authors: Hohenadel M, Thearle M, Grice B, Huang H, Dai M, Tao Y, Hunter L, Palaguachi G, Mou Z, Kim R, Tsang M, Haack K, Voruganti V, Cole S, Butte N, Comuzzie A, Muller Y, Baier L, Krakoff J, Knowler W, Yanovski J, Han J
      Int J Obes (Lond), 2014;38(8):1068-74.
      Species: Human
      Sample Type: Serum
    17. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C.
      Authors: Mera K, Uto H, Mawatari S, Ido A, Yoshimine Y, Nosaki T, Oda K, Tabu K, Kumagai K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Hidaka M, Eguchi S, Tsubouchi H
      BMC Gastroenterol, 2014;14(0):27.
      Species: Human
      Sample Type: Serum
    18. Short-term intake of a Japanese-style healthy lunch menu contributes to prevention and/or improvement in metabolic syndrome among middle-aged men: a non-randomized controlled trial.
      Authors: Inoue H, Sasaki R, Aiso I, Kuwano T
      Lipids Health Dis, 2014;13(0):57.
      Species: Human
      Sample Type: Serum
    19. Regional adipose tissue hormone/cytokine production before and after weight loss in abdominally obese women.
      Authors: You T, Wang X, Murphy K, Lyles M, Demons J, Yang R, Gong D, Nicklas B
      Obesity (Silver Spring), 2014;22(7):1679-84.
      Species: Human
      Sample Type: Plasma
    20. Adiponectin is a candidate biomarker of lower extremity bone density in men with chronic spinal cord injury.
      Authors: Doherty A, Battaglino R, Donovan J, Gagnon D, Lazzari A, Garshick E, Zafonte R, Morse L
      J Bone Miner Res, 2014;29(1):251-9.
      Species: Human
      Sample Type: Plasma
    21. Leptin is associated with vascular endothelial function in overweight patients with type 2 diabetes.
      Authors: Morioka T, Emoto M, Yamazaki Y, Kawano N, Imamura S, Numaguchi R, Urata H, Motoyama K, Mori K, Fukumoto S, Koyama H, Shoji T, Inaba M
      Cardiovasc Diabetol, 2014;13(0):10.
      Species: Human
      Sample Type: Plasma
    22. The type and quantity of dietary fat and carbohydrate alter faecal microbiome and short-chain fatty acid excretion in a metabolic syndrome 'at-risk' population.
      Authors: Fava, F, Gitau, R, Griffin, B A, Gibson, G R, Tuohy, K M, Lovegrove, J A
      Int J Obes (Lond), 2013;37(2):216-23.
      Species: Human
      Sample Type: Plasma
    23. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    24. A prospective study of circulating adipokine levels and risk of multiple myeloma.
      Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
      Blood, 2012;120(22):4418-20.
      Species: Human
      Sample Type: Plasma
    25. Alterations of leptin in the course of inflammation and severe sepsis.
      BMC Infect Dis, 2012;12(0):217.
      Species: Human
      Sample Type: Serum
    26. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
      Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
      Int J Obes (Lond), 2011;35(6):800-9.
      Species: Human
      Sample Type: Serum
    27. Adipocytokines and CD34 progenitor cells in Alzheimer's disease.
      Authors: Bigalke B, Schreitmuller B, Sopova K, Paul A, Stransky E, Gawaz M, Stellos K, Laske C
      PLoS ONE, 2011;6(5):e20286.
      Species: Human
      Sample Type: Plasma
    28. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
      Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
      Diabetes Care, 2010;33(8):1734-7.
      Species: Human
      Sample Type: Serum
    29. Leptin exacerbates sepsis-mediated morbidity and mortality.
      Authors: Shapiro NI, Khankin EV, van Meurs M
      J. Immunol., 2010;185(1):517-24.
      Species: Human
      Sample Type: Plasma
    30. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
      Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
      Diabetes, 2010;59(12):3049-57.
      Species: Human
      Sample Type: Plasma
    31. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
      Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
      Obesity (Silver Spring), 2010;18(11):2152-9.
      Species: Human
      Sample Type: Plasma
    32. Environmental tobacco smoke and cardiometabolic risk in young children: results from a survey in south-west Germany.
      Authors: Nagel G, Arnold FJ, Wilhelm M, Link B, Zoellner I, Koenig W
      Eur. Heart J., 2009;30(15):1885-93.
      Species: Human
      Sample Type: Plasma
    33. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
      Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
      AIDS, 2009;23(8):929-39.
      Species: Human
      Sample Type: Plasma
    34. Gene expression profiling of human skeletal muscle in response to stabilized weight loss.
      Authors: Larrouy D, Barbe P, Valle C, Dejean S, Pelloux V, Thalamas C, Bastard JP, Le Bouil A, Diquet B, Clement K, Langin D, Viguerie N
      Am. J. Clin. Nutr., 2008;88(1):125-32.
      Species: Human
      Sample Type: Plasma
    35. Diurnal variation of human sweet taste recognition thresholds is correlated with plasma leptin levels.
      Authors: Nakamura Y, Sanematsu K, Ohta R, Shirosaki S, Koyano K, Nonaka K, Shigemura N, Ninomiya Y
      Diabetes, 2008;57(10):2661-5.
      Species: Human
      Sample Type: Plasma
    36. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
      Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
      Thromb. Haemost., 2008;100(6):1123-9.
      Species: Human
      Sample Type: Plasma
    37. Positive energy balance is associated with accelerated muscle atrophy and increased erythrocyte glutathione turnover during 5 wk of bed rest.
      Authors: Biolo G, Agostini F, Simunic B, Sturma M, Torelli L, Preiser JC, Deby-Dupont G, Magni P, Strollo F, di Prampero P, Guarnieri G, Mekjavic IB, Pisot R, Narici MV
      Am. J. Clin. Nutr., 2008;88(4):950-8.
      Species: Human
      Sample Type: Plasma
    38. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
      Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
      Clin. Chem., 2008;54(7):1176-82.
      Species: Human
      Sample Type: Serum
    39. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
      Authors: Farese RV, Sajan MP, Yang H, Li P, Mastorides S, Gower WR, Nimal S, Choi CS, Kim S, Shulman GI, Kahn CR, Braun U, Leitges M
      J. Clin. Invest., 2007;117(8):2289-301.
      Species: Human
      Sample Type: Serum
    40. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
      Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
      Fertil. Steril., 2007;90(1):60-4.
      Species: Human
      Sample Type: Follicular Fluid
    41. Obesity, adipokines, and abdominal aortic aneurysm: Health in Men study.
      Authors: Golledge J, Clancy P, Jamrozik K, Norman PE
      Circulation, 2007;116(20):2275-9.
      Species: Human
      Sample Type: Serum
    42. The association of gastric leptin with oesophageal inflammation and metaplasia.
      Authors: Francois F, Roper J, Goodman AJ, Pei Z, Ghumman M, Mourad M, de Perez AZ, Perez-Perez GI, Tseng CH, Blaser MJ
      Gut, 2007;57(1):16-24.
      Species: Human
      Sample Type: Serum
    43. Panel of markers can accurately predict endometriosis in a subset of patients.
      Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
      Fertil. Steril., 2007;89(5):1073-81.
      Species: Human
      Sample Type: Serum
    44. Nitrosative stress predicts the presence and severity of nonalcoholic fatty liver at different stages of the development of insulin resistance and metabolic syndrome: possible role of vitamin A intake.
      Authors: Musso G, Gambino R, De Michieli F, Biroli G, Premoli A, Pagano G, Bo S, Durazzo M, Cassader M
      Am. J. Clin. Nutr., 2007;86(3):661-71.
      Species: Human
      Sample Type: Serum
    45. Altered hepatic expression of SREBP-1 and PPARgamma is associated with liver injury in insulin-resistant lipodystrophic HIV-infected patients.
      Authors: Lemoine M, Barbu V, Girard PM, Kim M, Bastard JP, Wendum D, Paye F, Housset C, Capeau J, Serfaty L
      AIDS, 2006;20(3):387-95.
      Species: Human
      Sample Type: Serum
    46. Plasma agouti-related protein levels in women with anorexia nervosa.
      Authors: Moriya J, Takimoto Y, Yoshiuchi K
      Psychoneuroendocrinology, 2006;31(9):1057-61.
      Species: Human
      Sample Type: Plasma
    47. Adiponectin and leptin in maternal serum, cord blood, and breast milk.
      Authors: Weyermann M, Beermann C, Brenner H, Rothenbacher D
      Clin. Chem., 2006;52(11):2095-102.
      Species: Human
      Sample Type: Serum
    48. Genetic regulation of the variation of circulating insulin-like growth factors and leptin in human pedigrees.
      Authors: Pantsulaia Ia, Pantsulaia I, Trofimov S, Kobyliansky E, Livshits G
      Metab. Clin. Exp., 2005;54(7):975-81.
      Species: Human
      Sample Type: Plasma
    49. Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.
      Authors: Chan DC, Watts GF, Ng TW, Uchida Y, Sakai N, Yamashita S, Barrett PH
      Clin. Chem., 2005;51(3):578-85.
      Species: Human
      Sample Type: Plasma
    50. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
      Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
      Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    51. Determinants of plasma leptin concentration in postmenopausal women.
      Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
      Mech. Ageing Dev., 2004;125(2):117-9.
      Species: Human
      Sample Type:
    Expand to show all 51 Citations
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     Human Leptin Quantikine ELISA Kit DLP00
    : Human Leptin Quantikine ELISA Kit [DLP00]

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    Sample TestedHuman serum

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