Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Lipin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693

Specificity

Detects human Lipin 1 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Lipin 1 Biotinylated Antibody

Lipin 1 in SK‑Mel‑28 Human Cell Line.

Lipin 1 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human Lipin 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3885) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Lipin 1 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SK-Mel-28 human malignant melanoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Lipin 1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Lipin 1

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.

Long Name

Phosphatidate Phosphatase LPIN1

Alternate Names

LPIN1

Entrez Gene IDs

23175 (Human); 14245 (Mouse); 313977 (Rat)

Gene Symbol

LPIN1

UniProt

Q14693

Additional Lipin 1 Products

Product Documents for Human Lipin 1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lipin 1 Biotinylated Antibody

For research use only

Related Research Areas

Obesity
Phosphatases and Regulators

Customer Reviews for Human Lipin 1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Lipin 1 Biotinylated Antibody and earn rewards!

Have you used Human Lipin 1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail