Lipin 1 in Human Adipocytes.
Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipin 1
Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has
been identified as an enzyme that regulates fat metabolism. There is
one human Lipin-1. In mice, two Lipin-1 isoforms have been reported,
alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 %
identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is
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