Human Lipocalin-2/NGAL Antibody

Catalog # Availability Size / Price Qty
MAB1757-SP
MAB1757-100
MAB1757-500
Detection of Human Lipocalin‑2/NGAL by Western Blot.
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Product Details
Citations (21)
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Reviews (2)

Human Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Lipocalin-1 or rmLipocalin-2. This antibody also recognizes human Lipocalin‑2/MMP‑9 complexes in Western blots under non-reducing conditions.
Source
Monoclonal Rat IgG2A Clone # 220310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL
Gln21-Gly198
Accession # P80188
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lipocalin‑2/NGAL antibody by Western Blot. View Larger

Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lipocalin-2/NGAL antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Lipocalin‑2/NGAL in Human Pancreas. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

References
  1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
  2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Human Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection
    Authors: Masafumi Okuda, Jun Inoue, Naoto Fujiwara, Tatsuyuki Kawano, Johji Inazawa
    Oncotarget
  2. Tumour stroma-derived Lipocalin-2 promotes breast cancer metastasis
    Authors: B Ören, J Urosevic, C Mertens, J Mora, M Guiu, RR Gomis, A Weigert, T Schmid, S Grein, B Brüne, M Jung
    J Pathol, 2016-04-29;0(0):.
  3. Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
    Authors: Barbara Mara Klinkhammer, Sonja Djudjaj, Uta Kunter, Runolfur Palsson, Vidar Orn Edvardsson, Thorsten Wiech et al.
    Journal of the American Society of Nephrology
  4. The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies
    Authors: Cheng, M;Zheng, Y;Fan, Y;Yan, P;Zhao, W;
    International immunopharmacology
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients
    Authors: F Lunardi, DI Abbrescia, L Vedovelli, F Pezzuto, F Fortarezza, GM Comacchio, V Guzzardo, P Ferrigno, M Loy, C Giraudo, AS Fraia, E Faccioli, F Braccioni, E Cozzi, D Gregori, GM Verleden, F Calabrese, FP Schena, F Rea
    Biomolecules, 2023-01-03;13(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
    Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
    Nature Communications, 2021-12-17;12(1):7333.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures
    Authors: I Zhang, P Lépine, C Han, M Lacalle-Au, CX Chen, R Haag, TM Durcan, D Maysinger
    Cells, 2020-11-07;9(11):.
    Species: Human
    Sample Types: Cell Lysates, Organoid, Whole Cells
    Applications: ICC, IHC, Western Blot
  8. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies
    Authors: S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain
    Ocul Surf, 2019-04-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Reactive astrocytes secrete lcn2 to promote neuron death.
    Authors: Bi, Fangfang, Huang, Cao, Tong, Jianbin, Qiu, Guang, Huang, Bo, Wu, Qinxue, Li, Fang, Xu, Zuoshang, Bowser, Robert, Xia, Xu-Gang, Zhou, Hongxia
    Proc Natl Acad Sci U S A, 2013-02-19;110(10):4069-74.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Lipocalin 2 expression is associated with aggressive features of endometrial cancer.
    Authors: Mannelqvist M, Stefansson I, Wik E, Kusonmano K, Raeder M, Oyan A, Kalland K, Moses M, Salvesen H, Akslen L
    BMC Cancer, 2012-05-06;12(0):169.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
    Authors: Mir SU, Jin L, Craven RJ
    J. Biol. Chem., 2012-03-14;287(18):14494-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  12. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
    Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
    J. Immunol., 2008-11-15;181(10):7420-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Urinary biomarkers predict brain tumor presence and response to therapy.
    Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
    Clin. Cancer Res., 2008-04-15;14(8):2378-86.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
    Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
    Arthritis Rheum., 2007-10-01;56(10):3326-35.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: Western Blot
  15. Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
    Authors: Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C
    Arthritis Rheum., 2007-06-01;56(6):1894-903.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot
  16. Pretreatment of carprofen impaired initiation of inflammatory- and overlapping resolution response and promoted cardiorenal syndrome in heart failure
    Authors: Veena Krishnan, David Booker, Gabrielle Cunningham, Jeevan Kumar Jadapalli, Vasundhara Kain, Amanda B. Pullen et al.
    Life Sciences
  17. The iron load of lipocalin-2 (LCN-2) defines its pro-tumour function in clear-cell renal cell carcinoma
    Authors: C Rehwald, M Schnetz, A Urbschat, C Mertens, JK Meier, R Bauer, P Baer, S Winslow, FC Roos, K Zwicker, A Huard, A Weigert, B Brüne, M Jung
    Br. J. Cancer, 2019-11-27;0(0):.
  18. Neutrophil Gelatinase-Associated Lipocalin and its Receptors in Alzheimer's Disease (AD) Brain Regions: Differential Findings in AD with and without Depression.
    Authors: Dekens DW, Naude PJ, Engelborghs S et al.
    J Alzheimers Dis
  19. Heterogeneity and Cancer-Related Features in Lymphangioleiomyomatosis Cells and Tissue
    Authors: Roderic Espín, Alexandra Baiges, Eline Blommaert, Carmen Herranz, Antonio Roman, Berta Saez et al.
    Molecular Cancer Research
  20. Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
    Authors: Emilly S. Villodre, Xiaoding Hu, Richard Larson, Pascal Finetti, Kristen Gomez, Wintana Balema et al.
    Molecular Oncology
  21. An experimental study of ascorbic acid effects in acute renal failure under general anesthesia
    Authors: Omur Ozturk, Sefer Ustebay, Huseyin Avni Eroglu, Murat Günay, Yasemen Adali, İlksen Donmez et al.
    Acta Cirurgica Brasileira

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Reviews for Human Lipocalin-2/NGAL Antibody

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Human Lipocalin-2/NGAL Antibody
By Anonymous on 10/12/2021
Application: IHC Sample Tested: Lung tissue Species: Human

Formalin-fixed lung sections


Human Lipocalin‑2/NGAL Antibody
By Anonymous on 02/24/2016
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human