Human Lipocalin-2/NGAL Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Lipocalin-1 or rmLipocalin-2. This antibody also recognizes human Lipocalin‑2/MMP‑9 complexes in Western blots under non-reducing conditions.
  • Source
    Monoclonal Rat IgG2A Clone # 220310
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL
    Gln21-Gly198
    Accession # P80188
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2-1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Lipocalin‑2/NGAL in Human Pancreas. Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

  • References:
    1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
    2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
    3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
    4. Devireddy, L.R. et al. (2001) Science 293:829.
    5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
    6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
    7. Flo, T.H. et al. (2004) Nature 432:917.
    8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  • Long Name:
    Neutrophil Gelatinase-associated Lipocalin
  • Entrez Gene IDs:
    3934 (Human); 16819 (Mouse); 170496 (Rat)
  • Alternate Names:
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Reactive astrocytes secrete lcn2 to promote neuron death.
    Authors: Bi, Fangfang, Huang, Cao, Tong, Jianbin, Qiu, Guang, Huang, Bo, Wu, Qinxue, Li, Fang, Xu, Zuoshang, Bowser, Robert, Xia, Xu-Gang, Zhou, Hongxia
    Proc Natl Acad Sci U S A, 2013;110(10):4069-74.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Lipocalin 2 expression is associated with aggressive features of endometrial cancer.
    Authors: Mannelqvist M, Stefansson I, Wik E, Kusonmano K, Raeder M, Oyan A, Kalland K, Moses M, Salvesen H, Akslen L
    BMC Cancer, 2012;12(0):169.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
    Authors: Mir SU, Jin L, Craven RJ
    J. Biol. Chem., 2012;287(18):14494-501.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  4. Urinary biomarkers predict brain tumor presence and response to therapy.
    Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
    Clin. Cancer Res., 2008;14(8):2378-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
    Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
    J. Immunol., 2008;181(10):7420-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
    Authors: Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C
    Arthritis Rheum., 2007;56(6):1894-903.
    Species: Human
    Sample Type: Urine
    Application: WB
  7. Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
    Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
    Arthritis Rheum., 2007;56(10):3326-35.
    Species: Human
    Sample Type: Synovial Fluid
    Application: WB
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Western Blot Human Lipocalin‑2/NGAL Antibody MAB1757-100
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Western Blot Human Lipocalin‑2/NGAL Antibody MAB1757-100
Western Blot: Human Lipocalin‑2/NGAL Antibody [MAB1757-100]

Summary

ApplicationWestern Blot
Sample TestedA549 human lung carcinoma cell line
SpeciesHuman

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