Detects human LRRC32/GARP in ELISAs. In direct ELISAs, no cross-reactivity with recombinant
mouse LRRC32 is observed.
Monoclonal Rat IgG2A Clone # 855151
Protein A or G purified from hybridoma culture supernatant
recombinant human LRRC32/GARP
Accession # Q14392
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Detection of LRRC32/GARP in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human LAP TGF‑ beta 1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2463P) and either (A) Rat Anti-Human LRRC32/GARP APC‑conjugated Monoclonal Antibody (Catalog # FAB6055A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature human LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine‑rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2, 3). Within the ECD, human LRRC32 shares approximately 80% aa sequence identity with mouse and rat LRRC32. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Human LRRC32 is identified as a lineage specific key receptor for human T cells. It is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6-8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
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Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
D11S833E; GARP; Garpin; D11S833Eleucine-rich repeat-containing protein 32; GARPGarpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32
Related Research Areas
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