Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MAO-A Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human MAO-A
Met1-Ser497 (predicted)
Accession # P21397

Specificity

Detects human MAO-A in direct ELISAs and Western blots. 

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MAO-A Antibody

Detection of Human MAO-A antibody by Western Blot.

Detection of Human MAO-A by Western Blot.

Western blot shows lysates of human placenta tissue, human liver tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MAO-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAO-A at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MAO-A Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Human placenta tissue, human liver tissue, and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAO-A

MAO-A (Monoamine oxidase type A and Amine oxidase [flavin-containing] A)  is a 60 kDa (predicted) member of the flavin monoamine oxidase family of proteins.  MAO- A is an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. It localizes to the outer mitochondrial membrane. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome, a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Over aa 1-497, human MOA-A shares 88% aa sequence identity with mouse MOA-A.

Long Name

Monoamine Oxidase A

Alternate Names

MAOA

Entrez Gene IDs

4128 (Human); 17161 (Mouse); 29253 (Rat)

Gene Symbol

MAOA

UniProt

P21397

Additional MAO-A Products

Product Documents for Human MAO-A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MAO-A Antibody

For research use only

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