Detection of Human MAO-A by Western Blot.
Western blot shows lysates of human placenta tissue, human liver tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MAO-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAO-A at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MAO-A (Monoamine oxidase type A and Amine oxidase [flavin-containing] A) is a 60 kDa (predicted) member of the flavin monoamine oxidase family of proteins. MAO- A is an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. It localizes to the outer mitochondrial membrane. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome, a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Over aa 1-497, human MOA-A shares 88% aa sequence identity with mouse MOA-A.
Monoamine Oxidase A
Entrez Gene IDs:
4128 (Human); 17161 (Mouse); 29253 (Rat)
amine oxidase [flavin-containing] A; EC 1.4.3; EC 188.8.131.52; MAOA; MAO-A; monoamine oxidase A; Monoamine oxidase type A
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