Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 408612
View all MKP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MKP-1
Met1-Cys367
Accession # P28562

Specificity

Detects human MKP-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MKP-2, rhMKP-3, or rhMKP-5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human MKP‑1 Antibody

MKP-1 antibody in Human Prostate by Immunohistochemistry (IHC-P).

MKP‑1 in Human Prostate.

MKP-1 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human MKP-1 Monoclonal Antibody (Catalog # MAB7168) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MKP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MKP-1

MKP-1, also known as PTPN10, DUSP1, and CL100, is an approximately 45 kDa Ser/Thr protein phosphatase that dephosphorylates p38 MAPK and JNK. Acetylation of MKP-1 enhances this activity and limits the inflammation-induced signaling downstream of TLR proteins. Within aa 1-367, human MKP-1 shares 97% aa sequence identity with mouse and rat MKP-1.

Long Name

MAP Kinase Phosphatase 1

Alternate Names

CL100, DUSP1, HVH1, MKP1, PTPN10

Entrez Gene IDs

1843 (Human); 19252 (Mouse); 114856 (Rat)

Gene Symbol

DUSP1

UniProt

P28562 (Human)

Additional MKP-1 Products

Product Documents for Human MKP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MKP‑1 Antibody

For research use only

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Protocols

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