MKP‑1 in Human Prostate. MKP‑1 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human MKP‑1 Monoclonal Antibody (Catalog # MAB7168) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MKP-1, also known as PTPN10, DUSP1, and CL100, is an approximately 45 kDa Ser/Thr protein phosphatase that dephosphorylates p38 MAPK and JNK. Acetylation of MKP-1 enhances this activity and limits the inflammation-induced signaling downstream of TLR proteins. Within aa 1-367, human MKP-1 shares 97% aa sequence identity with mouse and rat MKP-1.
MAP Kinase Phosphatase 1
Entrez Gene IDs:
1843 (Human); 19252 (Mouse); 114856 (Rat)
CL100; CL100MAP kinase phosphatase 1; dual specificity phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP1; EC 184.108.40.206; EC 220.127.116.11; HVH1; Mitogen-activated protein kinase phosphatase 1; MKP1; MKP-1dual specificity protein phosphatase 1; Protein-tyrosine phosphatase CL100; PTPN10; PTPN10serine/threonine specific protein phosphatase; VH1
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