Human MMP-19 Antibody

(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human MMP-19 in direct ELISAs.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MMP-19
    Leu229-Tyr508
    Accession # Q99542
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MMP-19 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for MMP-19 at approximately 57 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMP-19 in Human Melanoma. MMP-19 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-19

MMP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59 kDa member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490 amino acids (aa) in length. It contains an autocleavable 9 kDa propeptide (aa 19-97) plus a 411 aa mature region (aa 98-508). The mature region contains a Zn catalytic region (aa 103-256) plus four hemopexin-like domains (aa 293-508). There are three additional potential isoforms. One shows an 88 aa substitution for aa 300-508, a second contains a 12 aa substitution for aa 1-298, while a third possesses an alternative start site at Met80. Over aa 229-508, human MMP-19 shares 76% aa identity with mouse MMP-19.

  • Long Name:
    Matrix Metalloproteinase 19
  • Entrez Gene IDs:
    4327 (Human); 58223 (Mouse); 304608 (Rat)
  • Alternate Names:
    EC 3.4.24.-; matrix metallopeptidase 19; matrix metalloproteinase 18; matrix metalloproteinase 19; Matrix metalloproteinase RASI; Matrix metalloproteinase-18; matrix metalloproteinase-19; MMP-18; MMP18MMP-19; MMP19; MMP-19; RASI; RASI-1
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Isotype Controls
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Secondary Antibodies
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Goat IgG (H+L) APC-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 4 (Based on 2 reviews)

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Read what people are saying who have used Human MMP-19 Antibody.

We have 2 reviews tested in 2 species: Goat, Human.
We have 2 reviews tested in 2 applications: Functional Assay, Immunohistochemistry.

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Very Good
(2)
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Functional Assay
(1)
IHC
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Goat
(1)
Human
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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Functional Assay Human Anonymous 04/21/2017
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Summary

ApplicationFunctional Assay
Sample TestedCell culture supernatant,Plasma
SpeciesHuman

Other Experimental Details

Other Experimental Detailsin comparison: Animal models have become increasingly useful to examine the potential of MMP inhibitors to suppress aneurysm development and growth in vivo
Immunohistochemistry Human MMP-19 Antibody AF6790
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Very Good
 IHC Goat Anonymous 01/23/2017
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Image Details
Immunohistochemistry Human MMP-19 Antibody AF6790
Immunohistochemistry: Human MMP-19 Antibody [AF6790]

Summary

ApplicationImmunohistochemistry
Sample TestedHuman First trimester decidua
SpeciesGoat

Other Experimental Details

Other Experimental Detailssodium citrate antigen retrieval

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