Human MMP-19 Antibody

Catalog # Availability Size / Price Qty
AF6790
AF6790-SP
Detection of Human MMP-19 by Western Blot.
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Human MMP-19 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-19 in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-19
Leu229-Tyr508
Accession # Q99542
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MMP-19 by Western Blot. View Larger

Detection of Human MMP-19 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for MMP-19 at approximately 57 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP-19 in Human Melanoma. View Larger

MMP-19 in Human Melanoma. MMP-19 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-19

MMP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59 kDa member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490 amino acids (aa) in length. It contains an autocleavable 9 kDa propeptide (aa 19-97) plus a 411 aa mature region (aa 98-508). The mature region contains a Zn catalytic region (aa 103-256) plus four hemopexin-like domains (aa 293-508). There are three additional potential isoforms. One shows an 88 aa substitution for aa 300-508, a second contains a 12 aa substitution for aa 1-298, while a third possesses an alternative start site at Met80. Over aa 229-508, human MMP-19 shares 76% aa identity with mouse MMP-19.

Long Name
Matrix Metalloproteinase 19
Entrez Gene IDs
4327 (Human); 58223 (Mouse); 304608 (Rat)
Alternate Names
EC 3.4.24.-; matrix metallopeptidase 19; matrix metalloproteinase 18; matrix metalloproteinase 19; Matrix metalloproteinase RASI; Matrix metalloproteinase-18; matrix metalloproteinase-19; MMP-18; MMP18MMP-19; MMP19; MMP-19; RASI; RASI-1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-19 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human MMP-19 Antibody
By Anonymous on 04/21/2017
Application: Functional Assay Sample Tested: Cell culture supernatant,Plasma Species: Human

in comparison: Animal models have become increasingly useful to examine the potential of MMP inhibitors to suppress aneurysm development and growth in vivo


Human MMP-19 Antibody
By Anonymous on 01/23/2017
Application: IHC Sample Tested: Human First trimester decidua Species: Goat

sodium citrate antigen retrieval