Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 111433
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑7
Leu18-Lys267 (Ala230del)
Accession # NP_002414.1

Specificity

Detects the pro and active forms of human MMP-7 in Western blots. In dot blots, approximately 20% cross-reactivity with recombinant human MMP-8 is observed and no cross-reactivity with recombinant human MMP-1, -2, -3, -9, -10, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MMP‑7 Antibody

Detection of Human MMP-7 antibody by Western Blot.

Detection of Human MMP‑7 by Western Blot.

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human MMP-7 antibody by Simple WesternTM.

Detection of Human MMP‑7 by Simple WesternTM.

Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected for MMP‑7 at approximately 49 and 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human MMP-7 by Western Blot

Detection of Human MMP-7 by Western Blot

Zymography analysis for active MMP2 was performed with 4 μg of total protein of the conditioned medium of U87 (a) and primary IC cells (b) after 48 h exposure to JS-K (1–3 μM) on gelatin-containing PAGE. Western blot analysis for MMP2, MMP7, MMP9 and TIMP3 was performed for conditioned medium of U87 (a) and IC (b) cells with 4 μg of total protein as well as of 20 μg of total cell lysate of U87 (c) and IC cells (d) on SDS-PAGE. Conditioned media of knockdown and overexpression of ATF3 were examined for active MMP2 as well as the plasmid controls and uninfected U87 (e). 4 μg of protein of the supernatant (e) and 20 μg of cell lysates (f) were screened for MMP2, MMP7, MMP9 and TIMP3 by SDS-PAGE. Coomassie staining of the gels was used as a loading control for zymogram and for western blot of the supernatant, GAPDH was used as a loading control for cell lysates. Figures are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28250971), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Shows MMP‑7 Specificity Using Knockout Cell Line.

Western blot shows culture media collected from A549 human lung carcinoma parental cell line and MMP‑7 knockout A549 cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human MMP‑7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated secondary antibody. A specific band was detected for MMP‑7 at approximately 29.7 kDa (as indicated) in the culture media collected from parental A549 cell line, but is not detectable in the culture media collected from knockout A549 cell line. Primary antibody dilution used: 1 μg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for Human MMP‑7 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑7 (Catalog # 907-MP), see our available Western blot detection antibodies

Simple Western

20 µg/mL
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line, Human small intestine tissue, and CaSki human cervical carcinoma cell line

Western Blot

1 µg/mL
Sample: Capan‑1 human pancreatic adenocarcinoma cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, and Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

NP_002414.1

Additional MMP-7 Products

Product Documents for Human MMP‑7 Antibody

Certificate of Analysis

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Product Specific Notices for Human MMP‑7 Antibody

For research use only

Citations for Human MMP‑7 Antibody

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Protocols

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