Human MMP-9/TIMP-1 Complex DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human MMP-9 / TIMP-1 Complex ELISA Standard Curve
Preparation and Storage
Citations for Human MMP-9/TIMP-1 Complex DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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MMP-9 Levels and IMT of Carotid Arteries are Elevated in Obese Children and Adolescents Compared to Non-Obese
Authors: C Andrade, A Bosco, V Sandrim, F Silva
Arq. Bras. Cardiol., 2017;108(3):198-203.
Sample Types: Plasma
Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
Iran J Pathol, 2017;12(3):257-264.
Sample Types: Serum
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
BMC Cancer, 2013;13(0):598.
Sample Types: Plasma
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
Am. J. Respir. Crit. Care Med., 2005;172(5):559-65.
Sample Types: Sputum
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