Human MMP-9/TIMP-1 Complex DuoSet ELISA

Catalog # Availability Size / Price Qty
Ancillary Products Available
Human MMP-9 / TIMP-1 Complex ELISA Standard Curve
1 Image
Product Details
Citations (6)
Supplemental Products
Reviews (1)

Human MMP-9/TIMP-1 Complex DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
46.9 - 3,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-9 / TIMP-1 Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human MMP-9/TIMP-1 Complex DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. The diagnostic and prognostic potential of the EGFR/MUC4/MMP9 axis in glioma patients
    Authors: A Quesnel, N Coles, TM Polvikoski, GS Karagianni, C Angione, M Islam, AA Khundakar, PS Filippou
    Scientific Reports, 2022;12(1):19868.
    Species: Human
    Sample Types: Serum
  2. Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza
    Authors: S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost
    JCI Insight, 2021;0(0):.
    Species: Human
    Sample Types: BAL Supernatant
  3. MMP-9 Levels and IMT of Carotid Arteries are Elevated in Obese Children and Adolescents Compared to Non-Obese
    Authors: C Andrade, A Bosco, V Sandrim, F Silva
    Arq. Bras. Cardiol., 2017;108(3):198-203.
    Species: Human
    Sample Types: Plasma
  4. Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
    Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
    Iran J Pathol, 2017;12(3):257-264.
    Species: Human
    Sample Types: Serum
  5. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013;13(0):598.
    Species: Human
    Sample Types: Plasma
  6. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
    Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
    Am. J. Respir. Crit. Care Med., 2005;172(5):559-65.
    Species: Human
    Sample Types: Sputum


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human MMP-9/TIMP-1 Complex DuoSet ELISA

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human MMP-9/TIMP-1 Complex DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human MMP-9/TIMP-1 Complex DuoSet ELISA
By Jonatan Dereke on 10/24/2017
Sample Tested: EDTA Plasma

We ran human plasma samples at a 1:10 or 1:100 dilution for this assay. The intra-assay of variation reached 4.3%.