Human MMP-9/TIMP-1 Complex DuoSet ELISA

R&D Systems | Catalog # DY1449

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

46.9-3000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human MMP-9/TIMP-1 Complex DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all MMP-9/TIMP-1 Complex ELISA Kits »

Product Summary for Human MMP-9/TIMP-1 Complex DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-9 / TIMP-1 Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human MMP-9/TIMP-1 Complex DuoSet ELISA

Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

Kit Contents for Human MMP-9/TIMP-1 Complex DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Human MMP-9/TIMP-1 Complex DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP-9/TIMP-1 Complex DuoSet ELISA

For research use only

Citations for Human MMP-9/TIMP-1 Complex DuoSet ELISA

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  • Human MMP-9/TIMP-1 Complex DuoSet ELISA
    Name: Jonatan Dereke
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/24/2017
    We ran human plasma samples at a 1:10 or 1:100 dilution for this assay. The intra-assay of variation reached 4.3%.
    Human MMP-9/TIMP-1 Complex DuoSet ELISA DY1449

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