Human MMP-9/TIMP-1 Complex DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1449
Human MMP-9 / TIMP-1 Complex ELISA Standard Curve
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human MMP-9/TIMP-1 Complex DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-9 / TIMP-1 Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human MMP-9/TIMP-1 Complex DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. MMP-9 Levels and IMT of Carotid Arteries are Elevated in Obese Children and Adolescents Compared to Non-Obese
    Authors: C Andrade, A Bosco, V Sandrim, F Silva
    Arq. Bras. Cardiol., 2017;108(3):198-203.
    Species: Human
    Sample Types: Plasma
  2. Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
    Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
    Iran J Pathol, 2017;12(3):257-264.
    Species: Human
    Sample Types: Serum
  3. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013;13(0):598.
    Species: Human
    Sample Types: Plasma
  4. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
    Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
    Am. J. Respir. Crit. Care Med., 2005;172(5):559-65.
    Species: Human
    Sample Types: Sputum

FAQs

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Reviews for Human MMP-9/TIMP-1 Complex DuoSet ELISA

Average Rating: 5 (Based on 1 Review)

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Human MMP-9/TIMP-1 Complex DuoSet ELISA
By Anonymous on 10/24/2017
Application: Sample Tested: EDTA Plasma

We ran human plasma samples at a 1:10 or 1:100 dilution for this assay. The intra-assay of variation reached 4.3%.