Human/Mouse ADAMTS1 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse ADAMTS1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) ADAMTS4 and rhADAMTS15 is observed and less than 1% cross‑reactivity with rhADAMTSL1.2, rhADAMTSL2, rhADAMTS5, rhADAMTS8, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1
    Phe254-Cys725
    Accession # P97857
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse ADAMTS1 by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR‑3  human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
ADAMTS1 in Mouse Embryo.

ADAMTS1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium; dorsal ganglia cell bodies and processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAMTS1
ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha 2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.
  • References:
    1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
    2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
    3. Porter, S. et al. (2005) Biochem. J. 386:15.
    4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
    5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
    6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
    7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
    8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
    9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
    10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
    11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
    12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
  • Long Name:
    A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1
  • Entrez Gene IDs:
    9510 (Human)
  • Alternate Names:
    a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. ADAMTS-1 in abdominal aortic aneurysm
    Authors: E Vorkapic, M Folkesson, K Magnell, M Bohlooly-Y, T Länne, D Wågsäter
    PLoS ONE, 2017;12(6):e0178729.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo.
    Authors: Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B
    Sci Signal, 2016;9(451):ra103.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Galnt1 is required for normal heart valve development and cardiac function.
    Authors: Tian, E, Stevens, Sharon R, Guan, Yu, Springer, Danielle, Anderson, Stasia A, Starost, Matthew, Patel, Vyomesh, Ten Hagen, Kelly G, Tabak, Lawrence
    PLoS ONE, 2015;10(1):e0115861.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  4. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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