Human/Mouse ADAMTS1 Antibody

Detection of Human ADAMTS1 by Immunocytochemistry/Immunofluorescence

Immunohistochemical staining.(A) ADAMTS-1, (B) SMC marker alpha -actin, (C) macrophage marker in adventitial layer of human AAA aortas and (D) ADAMTS-1 in human non-aneurysmal control aortas. Staining of positive cells are seen in brown. The images are taken with 40X magnification and scale bar represent 50 μm. Red arrow indicates the same area. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human ADAMTS1 by Western Blot

Western blot analysis.(A) Expression of ADAMTS-1 and GAPDH in human AAA aortas (sample 1–6) and human non-aneurysmal control aortas (sample 7–11) and (B) quantification of ADAMTS-1 in relative values normalized to GAPDH. MWM: molecular weight marker indicating size in kDa. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ADAMTS1 by Western Blot

The metalloproteinase activity of ADAMTS1 is pivotal in stimulating the secretion of cleaved versican, activating the epidermal growth factor receptor (EGFR), promoting invasion, and conferring anoikis resistance in renal cell carcinoma (RCC) cells. A A schematic representation depicting two variants of the recombinant ADAMTS1 protein: one containing the metalloproteinase domain (ZnMc) and the other lacking it (TSP), along with a mutant ADAMTS1 expression construct (E402Q). B–E Treatment of Caki-1 cells with or without the recombinant ZnMc or TSP protein (40 nM) for 24 or 48 h. Subsequently, secretion of cleaved versican, activation of EGFR signal cascades, invasive abilities, and anoikis were respectively assessed using dot blot (B), western blotting (C), Matrigel invasion (D), and CCK8 assays. F–I Cleaved versican secretion (F), EGFR activation (G), invasive abilities (H), and anoikis (I) were evaluated in Caki-1 and 786-O cells after transducing wild-type (WT) ADAMTS1, ADAMTS1/E402Q, or a control vector. In D, E, H, and I, values are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the control group; #p < 0.05, ###p < 0.001, compared with the WT ADAMTS1-overexpressing group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39333870), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ADAMTS1 by Western Blot

ADAMTS1 expression promotes anoikis resistance of renal cell carcinoma (RCC) via inhibiting Bid, Bim, and Bak. A A western blot analysis was conducted to assess ADAMTS1 expression in Caki-1 and 786-O cells following transduction with either ADAMTS1 short hairpin (sh)RNA (left) or an ADAMTS1-expressing vector (right). B Cell viability of suspended Caki-1 and 786-O cells was evaluated using a CCK8 assay at 24 and 48 h post-stable overexpression (right) or knockdown (left) of ADAMTS1. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, compared with the control group. C Dot plot demonstrated the correlation between the single sample gene set enrichment analysis (ssGSEA) score of “negative regulation of anoikis” and ADAMTS1 expression in TCGA-KIRC patients. A Pearson correlation was performed to evaluate their association and significance. D Western blot analysis of intrinsic apoptosis-related proteins (Bad, Bcl-2, Bak, Bid, Bim, and PARP) in suspended Caki-1 cells manipulated with ADAMTS1. GAPDH served as a loading control. E and F Dissemination of RCC cells in zebrafish embryos. Caki-1 cells with ADAMTS1-knockdown were implanted into zebrafish embryos at 48 h post fertilization. Tumor cell dissemination was observed at 2 days post injection (dpi), with disseminated tumor foci indicated by white arrowheads on the trunk and end-tail (E). Integrated densities of Caki-1 metastatic tumor cells in the zebrafish trunk and end-tail at 2 dpi were quantified, with the mean value of the integrated density in the shCtl group set to onefold (F) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39333870), licensed under a CC-BY license. Not internally tested by R&D Systems.