Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

Catalog # Availability Size / Price Qty
AF8125
AF8125-SP
Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot.
5 Images
Product Details
FAQs
Supplemental Products
Reviews

Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
Glu21-Ser396
Accession # P06727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein A-IV/ApoA4 antibody by Western Blot. View Larger

Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot. Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Apolipoprotein A-IV/ApoA4 antibody by Western Blot. View Larger

Detection of Mouse Apolipoprotein A-IV/ApoA4 by Western Blot. Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Apolipoprotein A-IV/ApoA4 antibody in Human Small Intestine by Immunohistochemistry (IHC-P). View Larger

Apolipoprotein A-IV/ApoA4 in Human Small Intestine. Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Apolipoprotein A-IV/ApoA4 antibody in Human Ileum and Descending Colon by Immunohistochemistry (IHC-P). View Larger

Apolipoprotein A-IV/ApoA4 in Human Ileum and Descending Colon. Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human ileum (left panel) and descending colon (right panel) using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).

Simple Western Detection of Human Apolipoprotein A-IV/ApoA4 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Apolipoprotein A‑IV/ApoA4 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system.  Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption.  Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis.  ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well.  Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Entrez Gene IDs
337 (Human); 11808 (Mouse); 25080 (Rat)
Alternate Names
apo-AIV; apoA-IV; Apolipoprotein A4; Apolipoprotein AIV; Apolipoprotein A-IV; MGC142154; MGC142156

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody and earn rewards!

Have you used Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review