Human/Mouse Apolipoprotein A-IV/ApoA4 Antibody

Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot. Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Apolipoprotein A-IV/ApoA4 by Western Blot. Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein A-IV/ApoA4 in Human Small Intestine. Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Apolipoprotein A-IV/ApoA4 in Human Ileum and Descending Colon. Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human ileum (left panel) and descending colon (right panel) using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).

Detection of Human Apolipoprotein A‑IV/ApoA4 by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.