Human/Mouse Beclin 1 Antibody AF5295: R&D Systems

Human/Mouse Beclin 1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Beclin 1 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Beclin 1
    Met12-Gln239
    Accession # Q14457
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse Beclin 1 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, CH1 mouse B cell lymphoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5295) followed by HRP‑conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Beclin 1 at approximately 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Immunocytochemistry
Beclin 1 in HeLa Human Cell Line. Beclin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5295) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Beclin 1

Beclin 1 (Bcl-2 interacting coiled-coil protein; also know as ATG6) is a 60 kDa member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin 1 is widely expressed but abundantly found in mammary epithelium, glia and neurons. Human Beclin 1 is 450 amino acids (aa) in length and contains a Bcl-2 binding domain (aa 88‑150), a nuclear export signal motif (aa 180‑189), a coiled-coil region (aa 142‑270), and an evolutionary conserved domain (aa 244‑337). There is one potential alternate start site at Met88. Over aa 12‑239, human Beclin 1 is 98% aa identical to mouse Beclin 1.

  • Long Name:
    Beclin1, Autophagy-related
  • Entrez Gene IDs:
    8678 (Human); 56208 (Mouse); 114558 (Rat)
  • Alternate Names:
    ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30
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