Human/Mouse Cyclin B1 Antibody

Catalog #: AF6000
6 Citations Datasheet / COA / SDS

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AF6000
AF6000-SP

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Detection of Human Cyclin B1 by Western Blot.

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Cyclin B1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Cyclin B1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cyclin B1
Met1-Pro91
Accession # P14635

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cyclin B1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin B1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Cyclin B1 in Human Lymphoma. Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human Cyclin B1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclin B1 at approximately 67 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin B1

Cyclin B1 (also CCNB1 and G2/mitotic-specific cyclin-B1) is a member of the cyclin AB subfamily, cyclin family of proteins. Although its predicted MW is 50 kDa, it runs anomalously at 62 kDa in SDS-PAGE. Cyclin B1 associates with both CDK1 and 2 providing substrate specificity to a phosphorylating complex. A phosphor‑CDK1:Cyclin B1 complex is inactive and cytosolic during interphase. At the beginning of mitosis, CDK1 is dephosphorylated and activated, and the CDK1:Cyclin B1 complex initiates formation of the mitotic scaffold. Human Cyclin B1 is 433 amino acids (aa) in length. It contains two cyclin box folds (aa 201‑290 and 298‑383) and two substrate binding sites (aa 298‑342 and 343‑380). Phosphorylation occurs at Ser9, Ser35, Ser69, and Thr321. There is one potential alternative start site at Met252 and deletions of aa 363‑399 and 365‑433. Over aa 1‑91, human Cyclin B1 shares 63% aa identity with mouse Cyclin B1.

Entrez Gene IDs

891 (Human); 268697 (Mouse); 25203 (Rat)

Alternate Names

CCNB; CCNB1; Cyclin B1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Cyclin B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Recombinant Human Cytomegalovirus Expressing an Analog-Sensitive Kinase pUL97 as Novel Tool for Functional Analyses
Authors: N Krämer, M Schütz, UG Mato, L Herhaus, M Marschall, C Zimmermann
Viruses, 2022;14(10):.
Species: Human
Sample Types: Cell Lysate
Applications: Bioassay
Highly Conserved Interaction Profiles between Clinically Relevant Mutants of the Cytomegalovirus CDK-like Kinase pUL97 and Human Cyclins: Functional Significance of Cyclin H
Authors: M Schütz, R Müller, E Socher, C Wangen, F Full, E Wyler, D Wong, M Scherer, T Stamminger, S Chou, WD Rawlinson, ST Hamilton, H Sticht, M Marschall
International Journal of Molecular Sciences, 2022;23(19):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Functional Relevance of the Interaction between Human Cyclins and the Cytomegalovirus-Encoded CDK-Like Protein Kinase pUL97
Authors: M Schütz, M Steingrube, E Socher, R Müller, S Wagner, M Kögel, H Sticht, M Marschall
Viruses, 2021;13(7):.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation
Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy
Authors: FA Moretti, G Giardino, TCH Attenborou, AS Gkazi, BK Margetts, G la Marca, L Fairbanks, T Crompton, HB Gaspar
Scientific Reports, 2021;11(1):23221.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Enhanced Expression of the Key Mitosis Regulator Cyclin B1 Is Mediated by PDZ-Binding Kinase in Islets of Pregnant Mice
Authors: T Uesato, T Ogihara, A Hara, H Iida, T Miyatsuka, Y Fujitani, S Takeda, H Watada
J Endocr Soc, 2018;2(3):207-219.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
Authors: Porrello, Emanuela, Rivellini, Cristina, Dina, Giorgia, Triolo, Daniela, Del Carro, Ubaldo, Ungaro, Daniela, Panattoni, Martina, Feltri, Maria La, Wrabetz, Lawrence, Pardi, Ruggero, Quattrini, Angelo, Previtali, Stefano
J Exp Med, 2014;211(1):29-43.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot