Human/Mouse Cyclin B1 Antibody

Detection of Mouse Cyclin B1 by Western Blot

C3G inhibits the growth of B16-F10 cells in vivo. In situ tumor growth monitored via Xenogen IVIS imaging at different time points after implanting syngeneic B16-F10-luc tumors into male C57BL/6 mice (n = 10) either fed with control or C3G diet for 4 weeks. (A) Melanoma tumor growth was monitored in real-time via bioluminescent imaging of luciferase activity in live mice using the cryogenically cooled IVIS-imaging system from baseline to week 4 post implantation. (B) Graphical representation of tumor weight and tumor growth was monitored using Vernier calipers. (C) Photographic images of excised tumors were captured (n = 3). The largest tumor size in diameter is 2.0 cm. (D) Hematoxylin and eosin staining of tumor and capillaries are red (arrows). (E) Cleaved-caspase-3 was brownish in the cytoplasm (arrows) of the tumor and (F) Western blot analysis of caspase 3 and CCNB1 in C3G-treated mice tumors. (G), TUNEL staining to detect late apoptotic cells of the tumor (left panel) and C3G-treated mice tumors (Right panel). Late apoptotic cells had brownish nuclear regions (arrows). Differences with *p < 0.05, **p < 0.01, or ***p < 0.001 were considered statistically significant. Scale bar is 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696058), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Cyclin B1 by Western Blot

HCMV AD169-GFP replication kinetics in HFF WT and cyclin B1/T1 KO populations. HFFs were infected with HCMV AD169. Viral replication kinetics were determined by collecting viral supernatants at the indicated time points, and viral genome equivalents were determined by qPCR. Each value represents the mean ± SD of two independent biological replicates, each measured twice: (A) replication kinetics obtained for cyclin B1 KO cell populations A and B compared to WT (MOI 0.025); (C) replication kinetics obtained for cyclin T1 KO cell populations A, B and C compared to WT (MOI 0.01); (B,D) effectivity of cyclin B1 KO (cell populations A and B) and cyclin T1 KO (cell populations A, B and C), respectively, was verified by standard SDS-PAGE and Wb analysis using specific antibodies as indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36233116), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Cyclin B1 by Western Blot

HCMV AD169-GFP replication kinetics in HFF WT and cyclin B1/T1 KO populations. HFFs were infected with HCMV AD169. Viral replication kinetics were determined by collecting viral supernatants at the indicated time points, and viral genome equivalents were determined by qPCR. Each value represents the mean ± SD of two independent biological replicates, each measured twice: (A) replication kinetics obtained for cyclin B1 KO cell populations A and B compared to WT (MOI 0.025); (C) replication kinetics obtained for cyclin T1 KO cell populations A, B and C compared to WT (MOI 0.01); (B,D) effectivity of cyclin B1 KO (cell populations A and B) and cyclin T1 KO (cell populations A, B and C), respectively, was verified by standard SDS-PAGE and Wb analysis using specific antibodies as indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36233116), licensed under a CC-BY license. Not internally tested by R&D Systems.