Human/Mouse DGK-epsilon Antibody Summary
Accession # P52429
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse DGK‑ epsilon by Western Blot. Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DGK-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DGK-e at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DGK‑ epsilon in Human Brainstem. DGK-e was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human DGK-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DGK-epsilon (Diacylglycerol kinase epsilon) is a 65 kDa member of the eukaryocytic diacylglycerol kinase family of enzymes. It is a type III DGK that possesses only a C1/Cys-rich domain and a catalytic region, and is found in neurons and testis. DGK-epsilon specifically phosphorylates arachidonate-containing DAG, and may downregulate DAG signaling that results from inositol cycling. Human DGK-epsilon is 567 amino acids (aa) in length. It would appear to contain one transmembrane domain (aa 22-42), two C1 DAG-binding regions (aa 59-108 and 124-177) and one catalytic domain (aa 219-350). DGK-epsilon apparently can form intramembrane oligomers. Over aa 314-435, human DGK-epsilon shows 99% aa identity to mouse DGK-epsilon.
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Cell and Tissue Staining Kits
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