Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all DGK-epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK-epsilon
Asn314-Arg435
Accession # P52429

Specificity

Detects recombinant human DGK-epsilon in direct ELISAs. Detects human and mouse DGK-epsilon  in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse DGK‑ epsilon Antibody

Detection of Human and Mouse DGK-e antibody by Western Blot.

Detection of Human and Mouse DGK‑ epsilon by Western Blot.

Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DGK-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DGK-e at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DGK-e antibody in Human Brainstem by Immunohistochemistry (IHC-P).

DGK‑ epsilon in Human Brainstem.

DGK-e was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human DGK-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse DGK‑ epsilon Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brainstem

Western Blot

1 µg/mL
Sample: Caki‑2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGK-epsilon

DGK-epsilon (Diacylglycerol kinase epsilon) is a 65 kDa member of the eukaryocytic diacylglycerol kinase family of enzymes. It is a type III DGK that possesses only a C1/Cys-rich domain and a catalytic region, and is found in neurons and testis. DGK-epsilon specifically phosphorylates arachidonate-containing DAG, and may downregulate DAG signaling that results from inositol cycling. Human DGK-epsilon is 567 amino acids (aa) in length. It would appear to contain one transmembrane domain (aa 22-42), two C1 DAG-binding regions (aa 59-108 and 124-177) and one catalytic domain (aa 219-350). DGK-epsilon apparently can form intramembrane oligomers. Over aa 314-435, human DGK-epsilon shows 99% aa identity to mouse DGK-epsilon.

Long Name

Diacylglycerol Kinase, epsilon 64kDa

Alternate Names

DAGK5, DAGK6, DGKE, DGKepsilon

Entrez Gene IDs

8526 (Human); 56077 (Mouse)

Gene Symbol

DGKE

UniProt

P52429

Additional DGK-epsilon Products

Product Documents for Human/Mouse DGK‑ epsilon Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse DGK‑ epsilon Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human/Mouse DGK‑ epsilon Antibody

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Protocols

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