Human/Mouse Doublecortin/DCX Antibody

Catalog # Availability Size / Price Qty
AF10025-100
AF10025-SP
Human/Mouse Doublecortin/DCX Antibody in Western Blot
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Human/Mouse Doublecortin/DCX Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E.coli-derived recombinant human Doublecortin/DCX
Met116-Thr293
Accession # O43602
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Doublecortin/DCX by Western Blot. View Larger

Detection of Human Doublecortin/DCX by Western Blot. Western blot shows lysates of human motor cortex tissue, human hypothalamus tissue, and human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doublecortin/DCX at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Doublecortin/DCX  in Mouse Brain. View Larger

Doublecortin/DCX in Mouse Brain. Doublecortin/DCX was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Doublecortin/DCX

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Long Name
Doublecortin
Entrez Gene IDs
1641 (Human); 13193 (Mouse); 84394 (Rat)
Alternate Names
DBCN; Doublecortex; Doublecortin; Doublin; FLJ51296; Lissencephalin-X; LISX; Lis-X; X-linked (doublecortin)

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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