Doublecortin/DCX Antibody

R&D Systems | Catalog # AF10025

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Doublecortin/DCX Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Doublecortin/DCX
Met116-Thr293
Accession # O43602

Specificity

Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Doublecortin/DCX Antibody

Detection of Human Doublecortin/DCX antibody by Western Blot.

Detection of Human Doublecortin/DCX by Western Blot.

Western blot shows lysates of human motor cortex tissue, human hypothalamus tissue, and human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doublecortin/DCX at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Doublecortin/DCX antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Doublecortin/DCX in Mouse Brain.

Doublecortin/DCX was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Human/Mouse Doublecortin/DCX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10025) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Doublecortin/DCX Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Western Blot

1 µg/mL
Sample: Human motor cortex tissue, Human hypothalamus tissue, and Human hippocampus tissue

Reviewed Applications

Read 3 reviews rated 4 using AF10025 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Doublecortin/DCX

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Entrez Gene IDs

1641 (Human); 13193 (Mouse); 84394 (Rat)

Gene Symbol

DCX

UniProt

O43602

Additional Doublecortin/DCX Products

Product Documents for Doublecortin/DCX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Doublecortin/DCX Antibody

For research use only

Citations for Doublecortin/DCX Antibody

Customer Reviews for Doublecortin/DCX Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse Doublecortin/DCX Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Primate
    Verified Customer | Posted 08/05/2025
    Doublecortin/DCX Antibody AF10025
  • Human/Mouse Doublecortin/DCX Antibody
    Name: Sébastien Brot
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human iPSC-Derived Cerebral Organoids
    Species: Human
    Verified Customer | Posted 09/21/2023
    Doublecortin/DCX Antibody AF10025
  • Human/Mouse Doublecortin/DCX Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Primate
    Verified Customer | Posted 03/08/2022
    Doublecortin/DCX Antibody AF10025

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Protocols

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