Human/Mouse LRRN1/NLRR-1 Antibody

Catalog # Availability Size / Price Qty
AF4990
AF4990-SP
LRRN1/NLRR‑1 in Embryonic Mouse Somites.
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Citations (3)
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Human/Mouse LRRN1/NLRR-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human LRRN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NLRR-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRN1
Ser26-Ala631
Accession # AAH34947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LRRN1/NLRR‑1
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry LRRN1/NLRR‑1 in Embryonic Mouse Somites. View Larger

LRRN1/NLRR‑1 in Embryonic Mouse Somites. LRRN1/NLRR‑1 was detected in immersion fixed frozen sections of embryonic mouse somites (E9.5) using 10 µg/mL Sheep Anti-Human LRRN1/NLRR‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4990) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRN1/NLRR-1

LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.

Long Name
Leucine-rich Repeat Neuronal 1
Entrez Gene IDs
57633 (Human); 16979 (Mouse); 500280 (Rat)
Alternate Names
fibronectin type III, immunoglobulin and leucine rich repeat domains 3; FIGLER3; KIAA1497; leucine rich repeat neuronal 1; leucine-rich repeat neuronal protein 1; LRN; LRRN1; Neuronal leucine-rich repeat protein 1; NLRR1; NLRR-1

Product Datasheets

Citations for Human/Mouse LRRN1/NLRR-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Leucine-rich repeat neuronal protein-1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL
    Authors: B Liu, Y Zhang, Y Fan, S Wang, Z Li, M Deng, C Li, J Wang, R Ma, X Wang, Y Wang, L Xu, K Hou, X Che, Y Liu, X Qu
    Cancer Sci., 2019;110(7):2145-2155.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Molecular Logic of Spinocerebellar Tract Neuron Diversity and Connectivity
    Authors: M Baek, V Menon, TM Jessell, AW Hantman, JS Dasen
    Cell Rep, 2019;27(9):2620-2635.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma
    Sci Rep, 2016;6(0):32682.

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Secondary Antibodies

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