Human/Mouse LRRN1/NLRR-1 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human LRRN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NLRR-3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LRRN1
    Ser26-Ala631
    Accession # AAH34947
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LRRN1/NLRR‑1
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
LRRN1/NLRR‑1 in Embryonic Mouse Somites. LRRN1/NLRR‑1 was detected in immersion fixed frozen sections of embryonic mouse somites (E9.5) using 10 µg/mL Sheep Anti-Human LRRN1/NLRR‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4990) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRN1/NLRR-1

LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.

  • Long Name:
    Leucine-rich Repeat Neuronal 1
  • Entrez Gene IDs:
    57633 (Human); 16979 (Mouse); 500280 (Rat)
  • Alternate Names:
    fibronectin type III, immunoglobulin and leucine rich repeat domains 3; FIGLER3; KIAA1497; leucine rich repeat neuronal 1; leucine-rich repeat neuronal protein 1; LRN; LRRN1; Neuronal leucine-rich repeat protein 1; NLRR1; NLRR-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma
    Sci Rep, 2016;6(0):32682.
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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Sheep IgG Control

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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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