Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LRRN1/NLRR-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRN1
Ser26-Ala631
Accession # AAH34947

Specificity

Detects human LRRN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NLRR-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse LRRN1/NLRR‑1 Antibody

LRRN1/NLRR-1 antibody in Embryonic Mouse Somites by Immunohistochemistry (IHC-Fr).

LRRN1/NLRR‑1 in Embryonic Mouse Somites.

LRRN1/NLRR-1 was detected in immersion fixed frozen sections of embryonic mouse somites (E9.5) using 10 µg/mL Sheep Anti-Human LRRN1/NLRR-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4990) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of LRRN1/NLRR-1 by Western Blot

Detection of LRRN1/NLRR-1 by Western Blot

N1mAbs potentiates EGFR inhibitor-induced growth suppression. (A) NLRR1-stably expressing SH-SY5Y cells were treated with N1mAb 281 (25 μg/ml) and different concentrations of AG1478 (AG) for 72 h Data are represented as mean ± SD. Quantification of cell proliferation was performed by WST-8 assays. Data were normalized to the results for untreated cells and represented as percentage of control (mean ± SD). (B) AG1478-resistant A549 cells were treated with N1mAb 281 and different concentrations of AG1478 for 72 h (C) NLRR1-stably expressing SH-SY5Y cells were starved and treated with N1mAb 240 or 281 at 25 μg/ml for 3 h, followed by EGF treatment at the indicated concentration for 10 min. Cell lysates were subjected to western blot analyses. Arrowheads, glycosylated NLRR1. (D) MCF7 cells overexpressing NLRR1 were starved and treated with N1mAb 281 at 25 μg/ml for 3 h, followed by EGF treatment (1 ng/ml) for 10 min. The cell lysates were subjected to western blot analyses. (E) To check the effect of the long-term treatment of N1mAbs, SK-N-BE cells were incubated with N1mAbs (30 μg/ml) for 7 days with medium change every 2 days, and the cell lysates were subjected to western blot analyses. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34277416), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse LRRN1/NLRR‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse somites (E9.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Human LRRN1/NLRR‑1

Reviewed Applications

Read 1 review rated 5 using AF4990 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRN1/NLRR-1

LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.

Long Name

Leucine-rich Repeat Neuronal 1

Alternate Names

FIGLER3, LRN, NLRR-1, NLRR1

Entrez Gene IDs

57633 (Human); 16979 (Mouse); 500280 (Rat)

Gene Symbol

LRRN1

UniProt

AAH34947

Additional LRRN1/NLRR-1 Products

Product Documents for Human/Mouse LRRN1/NLRR‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse LRRN1/NLRR‑1 Antibody

For research use only

Citations for Human/Mouse LRRN1/NLRR‑1 Antibody

Customer Reviews for Human/Mouse LRRN1/NLRR‑1 Antibody (1)

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  • Human/Mouse LRRN1/NLRR-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer tissue
    Species: Human
    Verified Customer | Posted 09/10/2021
    Human/Mouse LRRN1/NLRR‑1 Antibody AF4990

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