Human/Mouse Periostin/OSF-2 Antibody

MAB3548 4 Citations Datasheet / COA / SDS

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MAB3548
MAB3548-SP

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Citations (4)

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Human/Mouse Periostin/OSF-2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 345613

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin isoform 2
Asn24-Gln811
Accession # NP_056599

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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Periostin/OSF‑2 in Human Osteocytes. Periostin/OSF-2 was detected in immersion fixed human osteocytes using Rat Anti-Human/Mouse Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB3548) at 10 µg/mL for 3 hours at room temperature. Cells were stained red and counterstained with DAPI (blue).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while theC-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672-812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787, and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts, and osteoblasts (2, 5, 7). Mature mouse OSF-2 is 98%, 92%, and 91% aa identical to rat, canine, and human OSF-2, respectively.

References

Clout, N.J. and D. Tisi (2003) Structure 11:197.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
Swiss-Prot Accession #:Q62009.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

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Citations for Human/Mouse Periostin/OSF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization
Authors: T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi
Mol Ther Nucleic Acids, 2017;6(0):279-289.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Histamine contributes to tissue remodeling via periostin expression.
Authors: Yang, Lingli, Murota, Hiroyuki, Serada, Satoshi, Fujimoto, Minoru, Kudo, Akira, Naka, Tetsuji, Katayama, Ichiro
J Invest Dermatol, 2014;134(8):2105-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Beta3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells.
Mol. Cancer, 2012;11(0):36.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
TRPC3-mediated Ca2+ influx contributes to Rac1-mediated production of reactive oxygen species in MLP-deficient mouse hearts.
Authors: Kitajima N, Watanabe K, Morimoto S, Sato Y, Kiyonaka S, Hoshijima M, Ikeda Y, Nakaya M, Ide T, Mori Y, Kurose H, Nishida M
Biochem. Biophys. Res. Commun., 2011;409(1):108-13.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot