Human/Mouse PLC-beta 1 Antibody

Catalog # Availability Size / Price Qty
AF4466
AF4466-SP
Detection of Human PLC‑ beta 1 by Western Blot.
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Human/Mouse PLC-beta 1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PLC-beta 1, but does not detect recombinant human (rh) PLC-beta 2, rhPLC-beta 3, or rhPLC-beta 4 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLC-beta 1
Lys27-Met245
Accession # Q9NQ66
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PLC‑ beta 1 antibody by Western Blot. View Larger

Detection of Human PLC‑ beta 1 by Western Blot. Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human/Mouse PLC‑ beta 1 antibody by Western Blot. View Larger

Detection of Human/Mouse PLC‑ beta 1 by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-beta 1

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 1 (Phospholipase C beta-1) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4, 5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. Alternative splicing results in two transcript variants encoding two isoforms, PLC-beta 1A and PLC-beta 1B. PLC-beta 1A is preferentially expressed in the cytosol, while PLC-beta 1B is predominantly expressed in the nucleus. The region used as immunogen is 100% identical in both isoforms.

Long Name
Phospholipase C beta 1
Entrez Gene IDs
23236 (Human); 18795 (Mouse); 24654 (Rat)
Alternate Names
EC 3.1.4.11; EIEE12; FLJ45792; inositoltrisphosphohydrolase; KIAA05811-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 1; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; Phosphoinositide phospholipase C-beta-1; phospholipase C, beta 1 (phosphoinositide-specific); Phospholipase C-beta-1; Phospholipase C-I; PI-PLC; PLC-154; PLC154,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1; PLCB1; PLCB1A; PLCB1B; PLCbeta 1; PLC-beta 1; PLC-beta-1; PLC-I; PLC-I1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; triphosphoinositide phosphodiesterase

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