The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 1 (Phospholipase C beta-1) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4, 5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. Alternative splicing results in two transcript variants encoding two isoforms, PLC-beta 1A and PLC-beta 1B. PLC-beta 1A is preferentially expressed in the cytosol, while PLC-beta 1B is predominantly expressed in the nucleus. The region used as immunogen is 100% identical in both isoforms.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human PLC-beta 1
Lys27-Met245
Accession # Q9NQ66
Lys27-Met245
Accession # Q9NQ66
Specificity
Detects human and mouse PLC-beta 1, but does not detect recombinant human (rh) PLC-beta 2, rhPLC-beta 3, or rhPLC-beta 4 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse PLC‑ beta 1 Antibody
Detection of Human PLC‑ beta 1 by Western Blot.
Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human/Mouse PLC‑ beta 1 by Western Blot.
Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse PLC‑ beta 1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human and mouse brain tissue
Sample: Human and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLC-beta 1
Long Name
Phospholipase C beta 1
Alternate Names
PI-PLC, PLC-154, PLC-I, PLCB1, PLCbeta 1
Gene Symbol
PLCB1
UniProt
Additional PLC-beta 1 Products
Product Documents for Human/Mouse PLC‑ beta 1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse PLC‑ beta 1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
