Human/Mouse/Rat Caspr2 Antibody

Catalog #: AF5145 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5145
AF5145-SP

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Detection of Human, Mouse, and Rat Caspr2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Caspr2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Caspr2 in direct ELISAs and human, mouse, and rat Caspr2 in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Caspr1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Caspr2 isoform 1
Pro29-Ala1262
Accession # Q9UHC6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Caspr2 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Caspr2 at approximately 160-170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Caspr2 in Mouse Brain. Caspr2 was detected in perfusion fixed frozen sections of mouse brain using 10 µg/mL Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr2

Caspr2 (CNTNAP2, Contactin-associated protein-like 2 and Cell recognition molecule Caspr2) is a 160-170 kDa glycoprotein, a member of the neurexin family of proteins. It is predominantly expressed in nervous system and likely play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Caspr2 and closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. Over aa 29-1262, human CASPR2 shares 94% aa sequence identity with mouse Caspr2.

Long Name

Contactin Associated Protein-like 2

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Alternate Names

AUTS15; Caspr2; CASPR2DKFZp781D1846; CDFE; Cell recognition molecule Caspr2; CNTNAP2; contactin associated protein-like 2; contactin-associated protein 2; contactin-associated protein-like 2; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; NRXN4Caspr2; PTHSL1