Detects human CCR5 in Western blots and human,mouse, and rat CCR5 in flow cytometry. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Monoclonal Mouse IgG1 Clone # CTC5
Protein A or G purified from hybridoma culture supernatant
CHO Chinese hamster ovary cell line transfected with human CCR5
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CCR5 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1802F) or (B) Mouse IgG1 Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL‑12 (Catalog # 419-ML) and 10 μg/mL Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1802F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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