Human/Mouse/Rat ERK1/ERK2 Antibody

  (9 citations)     
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Citations (9)
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat ERK1/ERK2.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ERK1/ERK2
    XM_055766 and NM_138957, respectively
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879‑KS) and Recombinant Human Active ERK2 (Catalog # 1230‑KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse ERK1/ERK2 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for ERK1/ERK2 at approximately 44 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

  • Long Name:
    Extracellular Signal-regulated Kinase 1/2
  • Alternate Names:
    ERK1/ERK2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Effect of electrical stimulation on neural regeneration via the p38-RhoA and ERK1/2-Bcl-2 pathways in spinal cord-injured rats
    Authors: MC Joo, CH Jang, JT Park, SW Choi, S Ro, MS Kim, MY Lee
    Neural Regen Res, 2018;13(2):340-346.
    Species: Rat
    Sample Type: Tissue Homegenates
    Application: WB
  2. The Role of PAR2 in TGF-?1-Induced ERK Activation and Cell Motility
    Authors: H Ungefroren, D Witte, C Fiedler, T Gädeken, R Kaufmann, H Lehnert, F Gieseler, BH Rauch
    Int J Mol Sci, 2017;18(12):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice
    Authors: BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli
    Front Cell Neurosci, 2017;11(0):313.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Western Blot
  4. Negative regulation of TGF-?1-induced MKK6-p38 and MEK-ERK signalling and epithelial-mesenchymal transition by Rac1b
    Authors: D Witte, H Otterbein, M Förster, K Giehl, R Zeiser, H Lehnert, H Ungefroren
    Sci Rep, 2017;7(1):17313.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014;35(3):339-50.
    Species: Human
    Sample Type: N/A
    Application: Not Specified
  6. A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromised migration in cultured vascular smooth muscle cells.
    Authors: Lo HM, Tsai YJ, Du WY, Tsou CJ, Wu WB
    J. Biomed. Sci., 2012;19(0):18.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  7. Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer.
    Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S
    Clin. Cancer Res., 2011;17(8):2260-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011;17(11):3619-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion.
    Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S
    J. Mol. Cell. Cardiol., 2009;46(5):612-20.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

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Isotype Controls
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Normal Rabbit IgG Control

Ctrl AB-105-C 46  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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