Human/Mouse/Rat Fascin Antibody

Catalog # Availability Size / Price Qty
AF7745
AF7745-SP
Detection of Human, Mouse, and Rat Fascin by Western Blot.
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Human/Mouse/Rat Fascin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, rat Fascin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Fascin-2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Fascin
Met1-Tyr493
Accession # Q16658
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.4 µg/mL
See below
Knockout Validated
Fascin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Fascin knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Fascin by Western Blot. View Larger

Detection of Human, Mouse, and Rat Fascin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat thymidine kinase-deficient embryonic fibroblast cell line. PVDF membrane was probed with 0.4 µg/mL of Sheep Anti-Human/Mouse/Rat Fascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fascin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.

Knockout Validated Western  Blot Shows Human Fascin Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Fascin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout HeLa cell line (KO). PVDF membrane was probed with 0.4 µg/mL of Sheep Anti-Human/Mouse/Rat Fascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

New adjunct appears with knockout cell line.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fascin

Fascin (that which creates fascicles [bundles] of actin; also known as 55 kDa actin-bundling protein, p55 and singed-like protein) is an intracellular 55-58 kDa member of the fascin family of proteins. It has a restricted expression pattern, being found in oligodendrocytes, select endothelium, cerebellar stellate neurons and blood, interdigitating, and thymic medullary dendritic cells. Fascin is found associated with actin in filopodia, and serves to coordinate and stabilize actin bundle formation, both in normal cells and tumor cells. In the latter cell type, filopodia have been renamed invadopodia, and their appearance is crucial for the creation of a stable platform that coordinates local matrix degradation. Human fascin is 493 amino acids (aa) in length. It contains an N-terminal fascin-like domain (aa 139-256) that contains part of one of two actin-binding sequences (aa 136-143), followed by two additional fascin-like domains (aa 260-378 and 383-493), the latter of which contains the second actin-binding sequence (aa 386-395). There are also two acetylation sites and two utilized phosphorylation sites at Ser38 and Ser39. Phosphorylation of the latter site inhibits fascin interaction with actin. At least two isoform variants may exist. One contains a 12 aa substitution for aa 427-493, while a second shows a deletion of aa 371-426. Full-length human fascin shares 97% aa sequence identity with mouse fascin. Two additional human fascins termed retinal and testis fascin have been identified. They are products of distinct genes and share 56% and 27% aa sequence identity with the standard (p55) fascin, respectively.

Entrez Gene IDs
6624 (Human); 14086 (Mouse)
Alternate Names
FAN1; FAN1,55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus); Fascin; FLJ38511; FSCN1; HSN; p55actin bundling protein; singed (Drosophila)-like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like Protein; SNL; SNLfascin

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