Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 426227
View all FKBP12/FKBP12.6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FKBP12.6
Gly2-Glu108
Accession # P68106

Specificity

Detects human FKBP12.6 in direct ELISAs. Detects human, mouse, and rat FKBP12 and FKBP12.6 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat FKBP12/FKBP12.6 Antibody

Detection of Human, Mouse, and Rat FKBP12 and FKBP12.6 antibody by Western Blot.

Detection of Human, Mouse, and Rat FKBP12 and FKBP12.6 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP12 and FKBP12.6 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Specificity of FKBP12 and FKBP12.6 Shown by Western Blot.

Specificity of FKBP12 and FKBP12.6 Shown by Western Blot.

Western blot shows recombinant human FKBP 12, recombinant human FKBP 12.6, recombinant human FKBP 13, recombinant human FKBP 25, recombinant human FKBP 38, and recombinant human FKBP 52. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP12 and FKBP12.6 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FKBP12 and FKBP12 by Immunohistochemistry (IHC-P).6 antibody in Human Brain by Immunohistochemistry (IHC-P).

FKBP12 and FKBP12.6 in Human Brain.

FKBP12 and FKBP12.6 was detected in immersion fixed paraffin-embedded sections of human brain (caudate putamen) using Rat Anti-Human/Mouse/Rat FKBP12/FKBP12.6 Monoclonal Antibody (Catalog # MAB4174) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat FKBP12/FKBP12.6 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate putamen)

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, Rat brain tissue, Recombinant human FKBP 12, and Recombinant human FKBP 12.6

Reviewed Applications

Read 1 review rated 5 using MAB4174 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP12/FKBP12.6

FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.

Long Name

12/12.6 kDa FK506 Binding Protein

UniProt

P68106

Additional FKBP12/FKBP12.6 Products

Product Documents for Human/Mouse/Rat FKBP12/FKBP12.6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat FKBP12/FKBP12.6 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Customer Reviews for Human/Mouse/Rat FKBP12/FKBP12.6 Antibody (1)

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  • Human/Mouse/Rat FKBP12/FKBP12.6 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 12/24/2021
    Human/Mouse/Rat FKBP12/FKBP12.6 Antibody MAB4174

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Protocols

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