Human/Mouse/Rat Glutaredoxin 3/GLRX3 Antibody

Catalog # Availability Size / Price Qty
AF7560
AF7560-SP
Detection of Human, Mouse, and Rat Glutaredoxin 3/GLRX3 by Western Blot.
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Human/Mouse/Rat Glutaredoxin 3/GLRX3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Glutaredoxin 3/GLRX3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Glutaredoxin 3/GLRX3
Asn126-Lys294
Accession # O76003
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Glutaredoxin 3/GLRX3 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Glutaredoxin 3/GLRX3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glutaredoxin 3/GLRX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7560) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Glutaredoxin  3/GLRX3 in Human Breast Cancer Tissue. View Larger

Glutaredoxin 3/GLRX3 in Human Breast Cancer Tissue. Glutaredoxin 3/GLRX3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse/Rat Glutaredoxin 3/GLRX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7560) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutaredoxin 3/GLRX3

GLRX3 (Glutaredoxin-3; also PICOT, PKC-theta-interacting protein, and Thioredoxin-like protein 2/TXNL2) is a 37-40 kDa member of the multidomain subgroup, monothiol glutaredoxin group of glutaredoxin/GTX proteins. It has widespread expression, but is not found ubiquitously. Cells known to express cytosolic GLRX3 are principally epithelial in type, and include breast and adrenal epithelium, pancreatic exocrine gland epithelium, and proximal tubule renal epithelium. Cardiac muscle may also expresses GLRX3. The function of GLRX3 is not clear. By inference, it is suggested to be involved in cell proliferation, and has also been associated with both PKC-theta regulation and MLP/muscle LIM protein-mediated cardiac contractility. Human GLRX3 is 335 amino acids (aa) in length. It contains an N-terminal thioredoxin-like domain (aa 2-117) that is followed by two PICOT homology domains (aa 144-236 and 237-335). The thioredoxin domain does not possess a typical disulfide, and it is suggested that this domain does not demonstrate redox activity. The PICOT domains will interact with intracellular proteins. GLRX3 exists as both a monomer and homodimer, with the homodimer incorporating two Fe/S clusters into their PICOT domains. These clusters serve as redox sensors within the cell. Over aa 126-294, human GLRX3 shares 98% aa sequence identity with mouse GLRX3.

Entrez Gene IDs
10539 (Human); 30926 (Mouse); 58815 (Rat)
Alternate Names
FLJ11864; GLRX3; GLRX4; Glutaredoxin 3; glutaredoxin 4; glutaredoxin 5; GRX3; GRX4; PICOT; PICOTbA500G10.4; PKC-interacting cousin of thioredoxin; PKCq-interacting protein; thioredoxin-like 2; TXNL2; TXNL3

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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